Effects Of Physiological Indexes And WRKY Genes Expression On Transgenic Maize Seedlings With CHB101 Gene Under NaCl Stress

Maize(Zea mays L.) is a type of crop used as food and fodder.Soil salinization and alkalization is an importantly environmental factor that restricts the yield of maize, so it is significant to breed the new varieties with salt resistance. ABA signaling pathway that is a hot spot of research in the field of plant science plays an important role in the abilities of salt and drought resistance. The gene CHB101 has been discovered in arabidopsis and is related with ABA signaling pathway. In the study, physiological and biochemical indexes of maize seedlings with CHB101 transferred in were measured. Real-time fluorescence quantification PCR was applied to quantify 29 WRKY genes performing sensitively. The difference between inbred lines with transgene CHB101 silencing and non-GMO inbred lines has been analyzed. Results are as follows:1. As the concentration of NaCl increasing,the indexes including phenotypic, root length, water content of above-ground part and underground part in maize inbred lines with gene CHB101 transferred in were better than those in non-GMO inbred lines.2. Proline content in leaves of transgenic maize inbred lines was higher than that of non-GMO inbred lines as the NaCl concentration increased to 200 mmol/L, while Proline content in roots of transgenic maize inbred lines was lower than that of non-GMO inbred lines; The rangeability of malonaldehyde(MDA) content in leaves of transgenic maize inbred lines was lower than that of non-GMO inbred lines with the NaCl concentration increasing, while no significant difference between rangeability of malonaldehyde(MDA) content in roots of transgenic maize inbred lines and rangeability of malonaldehyde(MDA) content in roots of non-GMO inbred lines was observed; Vitality of dehydrogenase in roots of transgenic maize inbred lines was higher than that in roots of non-GMO inbred lines under NaCl stress.3. Vitality of superoxide dismutase(SOD), peroxidase(POD), catalase(CAT) in roots and leaves of transgenic maize inbred lines was higher than that of SOD, POD, CAT in roots and leaves of non-GMO inbred lines as NaCl stress level increases.4. 29 WRKY genes sensitive to salt stress were quantified by employing the technology of Real-time fluorescence quantification PCR, 14 were up-regulated genes, 4 were down-regulated, 11 genes expression change was not significant.