The Physiological And Biochemical Changes And Disease Occurrence Of Corms Under Different Storage Methods On Gladiolus

Gladiolus is one of the important corm flowers in the world,Two gladiolus varieties Advance, Rose supreme bulbs as material, Adopt three kinds of storage methods, Measuring respiration, water and dry matter consumption of each storage period, and harmful pathogens,pathogenic bacteria types and harm during growth,Virus infection status,The purpose is to explore the corms during storage of physiological and biochemical changes and dormancy release,disease outbreak situation du ring field and storage.The results are as follows:(1)Two varieties were stored in three ways,The total water content and free amount of water decreased with the extension of storage time,The differences of varieties and storage methods were not obvious,Th e contents of bound water increased gradually.The respiratory rate of the bulb first fell to the lowest and then increased sharply,The highest point was reached at 8W and 12 W, and the time of the peak was different when the storage was different.The activity of PEPCase was increased after storage of 8W, and the changes of respiration rate were similar, and the differences were significant between cultivars and storage modes.The differences of POD activity between cultivars and storage were not obvious.Chang es of SOD activity and dormancy of closely related, after storage 4W, different varieties under different storage methods difference of SOD activity of larger, confirmed SOD activity can be as a predictor of bulb dormancy level indicator.The contents of endogenous GA3 were reached to the peak at the storage of 8W and after decreased sharply, the contents of different storage methods were varied,can also be used as the identification of the degree of dormancy parameters.By seeding emergence observation,two s pecies selection freezer storage are conducive to breaking dormancy and improve germination rate.(2)Gladiolus root rot during growth by microscopy and tieback experiments confirmed that the pathogen is Fusarium(Fusarium oxysporum); Corm rot during storage by microscopic examination, tieback experiment, ITS-r DNA molecular markers and BLAST homology alignment its pathogen are Penicillium,Basket bacteria subgenus, Talaromyces funiculosus, Different storage methods had the same disease,corm rot. the incidence have large differences,the treatment of the cold storage cabinet was significantly lower than other treatments.Using ELISA for samples suspected of carrying the virus detection, Does not detect CMV and TRV, Detect TMV, the detection rate is 8%.(3)The field investigation showed that the physiological dry tip of different varieties were significantly different, Pink Rose Supreme and red Advance dry tip most serious, purple Ostowte lightest.Choose dry tip severe varieties Rose Supreme to do experiments. Urea, potassium dihydrogen phosphate, ferrous sulfate, Pinxiu four trace elements as tested fertilizer fertilizer regulation experiment.The results showed that the effect of spraying ferrous sulfate was notable.