Residues And The Effect Of Microbial Community Structure In Gut Of Broiler Feedingcry1Ab/Ac Transgenic Rice

It’s an important part in safety evaluation of transgenic crops to detect residues and analyze the effects on community structure of microorganism in gut of broiler feeding cry1Ab/Ac transgenic rice. A feeding study was conducted to analyze the cry1Ab/Ac gene and protein residues and the effects on community structure of microorganism in gut of broilers and Rattus norvegicus fed genetically modified rice. The experiments results were as follows:1. There was no cry1Ab/Ac gene and protein residues in gut of broilers fed with Bt rice. The cry1Ab/Ac gene was not detected in jejunum, ileum, cecum and rectum of broilers fed transgenic rice for 21 days and 42 days. The cry1Ab/Ac gene was digested gradually in gut contents after 4 hours in vitro. The Cry1Ab/Ac protein was not detected by analysis of ELISA and Western blot.2. No significant effects were observed on community structure of intestinal microflora of broilers fed transgenic rice. The results of BIOLOG analysis and traditional culture method showed that there were no significant differences in the ability of using carbon source and functional diversity of microbe community, and the aboudance of the Enterobacteria, total aerobes, Lactobacilli, and total anaerobes in cecum of broilers fed Bt rice and isogenic for 42 days. Metagenome sequencing of 16 S r DNA showed that there were no significant differences between transgenic group and parent group in the main phylum,family and genus of gut. But microbe community of 42 days was richer than groups in 21 days, and obvious difference were observed in in the phylum,family and genus level3. There was no cry1Ab/Ac gene and protein residues in gut of Rattus norvegicus fed with Bt rice. The cry1Ab/Ac gene was not detected in jejunum, ileum and cecum of Rattus norvegicus fed transgenic rice for 50 days and 110 days. The cry1Ab/Ac gene was digested gradually in gut contents after 12 hours in vitro. The Cry1Ab/Ac protein was not detected by analysis of ELISA and Western blot. No significant effects were observed on community structure of intestinal microflora of Rattus norvegicus fed transgenic rice.4. No significant effects were observed on number of intestinal microflora of Rattus norvegicus fed transgenic rice. The results of traditional culture method showed that there were no significant differences in jejunum, ileum and cecum of Rattus norvegicus fed Bt rice and isogenic. There were some differences in gut of male rats fed in 50 days and 110 days.These results suggested that,there was no exogenous cry1Ab/Ac gene and protein residues in gut of broilers and Rattus norvegicus fed with Bt rice. No significant differences were observed betweenthe main intestinal microflora of broilers and Rattus norvegicus fed transgenic group and parent group. This study will provided data support to safety evaluation of transgenic crops, and laid the foundation to promote the industrialization of transgenic crops.