Isolation And Identification Of Inorganic Phosphorus Bacteria From Calcareous Soil Of Jinzhong City In Shan Xi Province

Explore Jinzhong City calcareous soil rhizosphere species, solubilizing ability and solubilizing mechanism solubilizing bacteria, according to the findings in order to filter out the highly efficient and stable solubilizing strains for producing a strong biological activity of soil phosphate solubilizing provide good strain, thus achieving in calcareous soil to replace phosphate solubilizing bacteria, reduce the amount of fertilizer to increase crop yield, reduce soil pollution. Calcareous soil is a general term which profiles contain soil calcium carbonate or bicarbonate of lime substance. In China mainly distributed in the north and northwest sub-humid, semi-arid and arid regions, the soil parent materials are mostly loess-like sediments and limestone weathered material. The distribution of calcium carbonate in the profile, depending on the soil conditions, some soil uniformly mixed, some soil porosity along with mycelium or film-like white precipitate present some form of hard tuberculosis, as well as in certain the formation of carbonate accumulation layer depth. Thus, calcareous soil phosphorus has a strong fixed effect, which is very low concentration of phosphorus in the soil solution, and low mobility. Mobility and closely related to the water content of soil phosphorus. The calcareous soils in arid little precipitation and semi-arid regions of phosphorus are due to low soil moisture and weaken the diffusion and root growth of roots. Phosphorus deficiency is the large variety of plant growth in calcareous soil limiting factor. Soil phosphorus deficiency has become a major limiting factor for crop growth in these areas. The traditional method of soil improvement fertilization alone often can not effectively solve the problem of soil phosphorus deficiency.This article from the separation of inorganic phosphorus in calcareous soil bacteria, screening start. Farmland, vegetable greenhouses Jinzhong eight counties and other places for collecting soil samples, soil samples collected for bacterial isolation, screening and identification, and through access to relevant information, on which the bacteria, yeasts and fungi and other bacteria class to do a detailed understanding screened six growing well and relatively pure inorganic phosphate bacteria. Then strain the inorganic and organic phosphorus solubility measurement, staining and morphological, physiological and biochemical identification. By morphological, physiological and biochemical reactions and bacterial identification manual, initially identified two belong to the genus Pseudomonas, four belonging to the genus Bacillus. Study on phosphorus dissolved inorganic phosphorus ability of bacteria found: six strains of bacteria, inorganic phosphorus dissolving calcium phosphate capacity W5 is the largest of the six strains, W12 is minimal, phosphate solubilization capacity between 86.34 ~ 190.86 mg / l; and for dissolving phosphate rock phosphate little capacity gaps, the largest is W12, the smallest is the W15, phosphate solubilization capability between 3.75 ~ 20.79 mg / l. Six strains of inorganic phosphate bacteria organophosphate solubilizing also have some ability, or less, far less strong for dissolving calcium phosphate capacity. Solubilizing ability of egg yolk lecithin between 5.79 ~ 28.97 mg / l; soybean lecithin solubilizing capacity between 0.80 ~ 24.32 mg / l. Isolation and Identification of calcareous soils by inorganic phosphate bacteria, better screening solubilizing strains in order to change the status of soil phosphorus deficiency, make full use of microorganisms in the soil and provide a theoretical basis for phosphorus.