Resistant Genes Detection, Identification Of Indica-Japonica Type And Prediction Of Heterosis In Rice Varieties

It is an effective method of controlling rice blast and brown plant-hopper to breeding and utilizing resistant rice cultivars. The rice blast resistance genes Pi-b, Pi-k, Pi-ta and brown plant-hopper resistance genes bph2, Bph3 of 2 male sterile lines and their 16 restorer lines were detected by gene functional marker. It laid the foundation for the gene pyramiding and breeding the excellent rice cultivars with broad spectrum and durable resistance to rice blast and brown plant-hopper by molecular identification to related resistance genes of rice cultivars. In this paper the combination for apolegamy was studied on the InDel markers, with the independent selection Zhejing 4A and 22 restorer lines and combinations, and with the independent selection Zhejing 5A and 22 restorer lines and combinations as the test material. Analyzed the relationship between the parents’s genetic distance, and studied the feasibility of InDel markers genetic distance to predict heterosis based on production traits of the combinations.1. The results showed that there N41, N49, N58, R23, R45, R47, R48, R53, R60, R68, R72 total of 11 tested materials contained gene Pi-b, N41, N51, N58, R28, R32, R45, R47, R48, R53, R60, R67, R68, R72 total of 13 tested materials contained gene Pi-k, among them 9 tested materials contained gene Pi-b as well as Pi-k, and Zhejing 4A, Zhejing 5A and R47 total of 3 tested materials contained gene bph2 in the rice blast resistance gene detection and the brown planthopper,2. Using polymorphism of the materials in this study on the InDel site as the original data, computing the gene frequency of indica or japonica identification subspecies properties and NTSYS cluster analysis and principal component analysis. The results showed that 2 male sterile lines are divided in japonica group. Zhejing restorer 14 of 22 restorers are divided in indica or indica dine, which combinations are in the group of inter indica-japonica type or japonica cline. Zhejing restorer 27 of 20 restorers are divided in japonica or japonica cline and Zhejing restorer 436 of 20 restorers are divided in inter indica-japonica type, which combinations gathered in the group of japonica or japonica cline also.3. Access to genetic distance between the parents of hybrids, computing control heterosis of combinations by yongyou 9 as control, and analysis the relationship of InDel genetic distance with the control heterosis of production traits. The results of two parts show that the InDel genetic distance and total grains per panicle, filled grain number per panicle, yield per plant were extremely significant positive correlation; Genetic distance and panicles were significant correlated; and showed not significant positive correlation with seed setting rate. Genetic distance and grain weight were not significant correlated in the 22 combinations of; and showed significantly negative correlation in the 24 combinations of Zhejing 5 A as female parients. Panicles, total grains per panicle and filled grain number per panicle are consistent with yield per plant, InDel markers can be used to predict the rice yield per plant.