Transcriptome Analysis Of Scutellaria Baicalensis Georgi Through High-throughput Sequencing

Scutellaria baicalensis Georgi for the Labiatae, another name is mountain tea root or root soil gold tea. In this paper, we choose root, branch, flower and leaf organs of Scutellaria baicalensis Georgi as the experimental material, and use the high-throughput sequencing technology (Illumina HiSeqTM2500 sequencing technology) to sequence the transcriptome of Scutellaria baicalensis Georgi. Then we systematic analyze the sequencing results in the bioinformatics way, and it will laid a solid foundation for the future development of genomics and molecular biology research about Scutellaria baicalensis Georgi.The conclusions of this study are as follows:(1) After the extraction of the sample RNA about Scutellaria baicalensis Georgi, preparation of the cDNA library, Illumina HiSeqTM2500 sequencing, filtration of the failure sequence, and assembly steps, eventually, we got 53353 of Scutellaria baicalensis Georgi Unigene.which total length is 42572289 nt, an average length is 797.94 nt and N50 length is 1467 nt.(2) Compared with the COG, GO, KEGG, Swiss-Prat, NR database by using the Blast software, the obtained Unigene respectively matches 10756,21950,7416,20339,29288 of the mentioned database. Comparing with the COG database, we divided it into 25 different gene functional annotation.(3) GO and Pathway enrichment analysis of the Unigene was made to get 57 different functional categories and 116 metabolic pathways. Homology compared with the six enzymes which were play the main role in the flavonoids synthesis pathway of Scutellaria baicalensis Georgi, the results showed that there were 30 Unigene may be involved in the coding.of flavonoids synthesis pathway.(4) We made a CDS forecast to got a total of 53082 CDS by using the GetOrf software.(5) By using the SSR analysis, we got a total of 5658 SSR markers.The mono-nucleotide repeats, di-nucleotide repeats and tri-nucleotide repeats which SSR type are majorly distributed account for 98.89% of the total markers, the left quad-nucleotide repeats, penta-nucleotide repeats, hexa-nucleotide repeats account for 1.11%.(6) Analyzing gene expression in the sample quantity,98 Unigene FPKM value is greater than 600. 314 Unigene FPKM value less than 0.2, the sequencing results are good.