Development And Pharmacokinetics Study Of HPBV-ceftiofur Sustained-release Preparation

Objective:The infectious diseases in the livestock breeding industry represent a significant drawback that generates substantial economic loss and have led to the indiscriminate use of antibiotics. The formulation of polymeric microparticles loaded with antibiotics for veterinary use can:reduce the number of required doses; protect the drug from inactivation; reduce the cost of animal drugs; maintain a sustained-release of the antibiotic drug at effective levels; and improve the cure rate. Accomplishing all of these goals would have a significant economic, animal health, food safety, and quality of life of the people impact on the livestock breeding industry.Method:In this work, we formulated ceftiofur-loaded PHBV microparticles, dosed SD rats by intramuscular injection, detected the plasma concentration, and calculated the DMPK parameters, as bioavailability and so on.Result:Preparations made, with 2 mg/mL,4 mg/mL,8 mg/mL three dosage preparation is completed, before the animal drug delivery, sampling results for the detection of HPLC-UV obtained were 95.9%, 96.2%,93.1%, among the system confidence range (80%-120%), the quality of the preparation meets the requirement of use. In the calculation results of biological samples for Pharmacokinetic parameters, the T1/2 are basic for more than 30 hours, the ratio of total area under the curve area calculation for 24 hours under the curve is more than 120%, explain the sustained release effect of the preparation of the drug was significantly.Conclusion:Our results show that, PHBV loaded ceftiofur particles is no question the quality of preparation, has potential; and can delay the in vivo release and elimination process, so can improve livestock infectious disease treatment effect.