Different Nitrogen Source Fodder Add Low Levels Of Condensed Tannin Influence In Vitro Fermentation

This experiment with soybean meal and alfalfa hay as nitrogen source fodder, the substrate to do add low levels of condensed tannin, in vitro fermentation experiment was carried out, the different nitrogen source fodder add low levels of condensed tannin on rumen fermentation parameters, the influence of in vitro for condensed tannins in protecting the rumen bypass protein offers scientific basis for further application.In vitro experiment:to study the different nitrogen source fodder add low levels of condensed tannin influence on rumen fermentation parameters in vitro.Substrate respectively soymeal fodder and alfalfa hay fodder 2 g each, adding 0.3%,0.6% and 0.3% respectively of the low levels of condensed tannin, marked as control group (0%), soybean meal, soybean meal group Dl (0.3%), soybean meal group (0.6%), soybean meal, D2 D3 (0.9%) and alfalfa hay group control group (0%), alfalfa hay M1 M2 (0.3%), alfalfa hay group (0.6%), M3 alfalfa hay group (0.9%). In vitro culture determine the treatment group after 24 h, pH value, gas production, ammonia nitrogen, bacteria protein, volatile fatty acids.Results show that the soybean meal fodder and fodder adding alfalfa hay low condensed tannins, with the increase of condensed tannin content and the extension of fermentation time, pH value is on the decline. Soybean meal group at 6 h, significantly below Dl, D2 and D3 group group. In alfalfa hay when adding different amount of low levels of condensed tannin, within each sampling time point group differences were not significant. Significant difference in the process of gas production in the whole, along with the increase in condensed tannin content decreases, the soybean meal group group of gas production, gas production rate is higher than alfalfa hay in plus 0.9% soybean meal and alfalfa hay in the low level of gas production minimum when condensed tannin, low levels of condensed tannin is to reduce the effect of gas production. Adding low levels of condensed tannin in soybean meal fodder and fodder, alfalfa hay with the extension of time, the ammonia nitrogen content showed a trend of increase, condensed tannins, with the increase of adding amount of ammonia nitrogen content, to reduce the growth rate of the growth rate has a tendency to pick up when the 24 h. Alfalfa hay fodder and meal fodder add low levels of condensed tannin, D1, D3 group and M1, M3 group of bacteria protein content showed a trend of decline after rising first, D2 and M2 group of bacteria protein content declined. The total volatile fatty acid and acetic acid content is the lowest is M3 group, followed by M2 and M1 group, the whole process of in vitro fermentation, were significantly lower than that of soybean meal in the three treatment groups, but with the extension of time, the difference is gradually reduce. Two kinds of nitrogen source in feed production of propionic acid with the highest group is D3 group and M3, respectively.Different nitrogen source fodder low levels of condensed tannin adding quantity, the size of the have a certain influence on rumen fermentation parameters. Along with the increase in low levels of condensed tannin content, pH value falls slowly, gas production rate decreased significantly, the content of. ammonia nitrogen was slowly rising trend, the bacteria protein content and total volatile fatty acid and acetic acid content are a downward trend, the content of propionic acid with low levels of condensed tannin content increased with increasing. Compared with soybean meal, alfalfa hay after adding low levels of condensed tannin in all kinds of volatile fatty acids is low, and volatile fatty acids keep in a stable state, low levels of condensed tannin content have less effect on the volatile fatty acids.