The Restorer Lines Improvement Of Bt Transgenic Rice

In this study, TakingJiangxi agricultural University excellent restorer chang hui121, chang hui 606, chang hui 891, chang hui T025 for female parent and respectively taking transgenic restorer varieties Ming hui 63 cry1 C, Ming hui 63 cry2 A, Ming hui 63 cry2 A +1 C, Ming hui 63 cry1 Ab + 2A, Ming hui 63 cry1 Ab + 1 C for male parent, we get F1 by the artificial hybridization. And then respectively taking chang hui121, chang hui 606, chang hui 891, chang hui T025 as the recurrent parent for backcross 7 times, then selfing twice,using the strip method in this process, the identification of indoor artificial infestation insect resistance and agronomic traits combined pedigree selection method to obtain transgenic insect resistance with rice restorer line 20 a. The breed of the insect-resistant transgenic lines were restored and Sterile lines 1892 S, Y58 S, wide accounting 63 S, red176 S, Tai Feng A, Wu Xiang S hybridized to obtain 80 combinations.The trial of 20 insect nurtured restorers have five insect restorers higher than recurrent parent. M06 was higher among Chang Hui 606, M11, M13, M14 Chang Hui higher than 891, M18 higher than Chang Hui T025. In insect experiments, nurtured by20 restorers insect pest levels are highly resistant to HR, and 121 as a control Chang Hui Chang Hui 606, 891 Chang Hui Chang insect levels were restored T025 anti- MR,cultivate new insect pest restorers significantly higher capacity. These results suggest that M6, M11, M13, M14, M18 which five varieties not only have good comprehensive agronomic traits, and there is a relatively strong insect ability.80 insect varieties using a combination of the test strip assay, were significantly positive, The yield of 29 varieties was higher than Ⅱ You 838. Yield per plant was higher as the female parent to Y58 S five insect combinations have N06-2, N07-2, N08-2,N09-2, N10-2; and as the female parent to Tai Fung A 20 a pest combinations have N01-2, N02-2, N03-2, N04-2, N05-2, N06-4, N07-4, N08-4, N09-4, N10-4, N11-1, N12-1, N13-1, N14-1, N15-1, N16-4, N17-4, N18-4, N19-4, N20-4. More than 80 insect combinations have 64 combinations of seed rate was higher.Combined with good recovery system on the overall performance of GCA and SCA restorer of agronomic traits and nurtured with the CMS line with the group ’s analysis found that the breed has restorer Chang Hui 606Cry1 C, Chang Hui 891Cry1 Ab + 2A,Chang Hui T025Cry1 C + 2A.