| Objective:This study was to investigate the prevalence of Eperythrozoonosis in Changzhi, Shanxi province, and we got the rate of infection though the laboratory diagnosis, so that provided evidence for the prevention and the formulation of relevant policies.Methods:we have researched epidemiological, clinical symptoms in the 11 counties of Changzhi, Shanxi province. By the method of Giemsa staining, Rick’s staining technique, we attached red cell body disease epidemiology investigation, and to establish a PCR diagnostic method in the original of this diseaseResults:1. According to the surveys of different cities in Changzhi, we found that the rate of pigs infected with Eperythrozoon was the highest in Wuxiang in all areas of investigation, reaching 80%, and Qin Country and Pingshun County of Eperythrozoonosis in swine infection rate was the lowest at 68%; the infection rate was higher in the summer than in winter, which reached to 70%; the infection rate of Eperythrozoon was higher in farmers than in scaled farms; both male and female pigs infection rate were almost the same; the infection rate of eperythrozoonosis in weaned piglets was very high, while the suckling piglets was at the lowest.2. In this survey, from the clinical symptoms and pathological changes, we can see that sick pigs infected with porcine eperythrozoonosis often occurred suddenly and presented a serious symptoms, such as lethargy, weakness, loss of appetite, increased body temperature, skin flushing or pale, visible mucous membranes pale or yellow dye, emaciation, anemia, etc.The swines were dissected after died and visible anemia and jaundice was obvious. Other changes were as follows:visual mucosa showed yellow or pale color, the blood was thin as water, coagulation adverse, subcutaneous fat stained yellow, muscle pale and edematous. Myocardial relaxation, pericardial effusion, the inner existed light red liquid, the fat of coronary yellow dyed; liver enlargement, crisp, yellow and accompanied by bleeding point, full of bile gallbladder; spleen enlargement become soft, the edge of a bleeding point, generalized lymphadenopathy; pulmonary edema without elasticity, there is bleeding; renal enlargement, color light, gray white or yellow white necrotic foci; mesentery lymph edema, hemorrhage.3. Through the microscope, we can see that Eperythrozoon suis were short rod shaped, spherical or toroidal under light microscope, the infected red blood cell loses stereo shape, margin irregularly, refraction; from Giemsa staining under light microscope we can see the red blood cells of normal structure integrity, no surface body attached. In positive cases, we can observed in the red cell surface they are attached, when trimming, there was refraction and red blood cell edge not smooth; from Wright’s staining, we can see normal intact, the surface was smooth, and without attachment bodies. In positive cases, cells and Eperythrozoon were dyed dark blue purple, attached to the surface of red blood cell, and the color is more deeper in Eperythrozoon.4. The PCR product results showed that the detected samples have the target band, and the size was about 782bp, consistent with expected amplification size.Conclusion:1. Based on the investigation of pigs in different counties of Changzhi area, we got the initial grasp of the epidemic situation in Changzhi city of Eperythrozoonosis in swine, the infection rate was the highest in Wuxiang County, and the infection rate was the lowest in Qin County and Pingshun County.2. This study established laboratory methods for the diagnosis of Eperythrozoonosis in swine. By comparing the different staining method, the smear staining method may lead to a false positive, and the final results should be based on the PCR results.3. Pigs infected with Eperythrozoon infection was mainly recessive infection. Routinely, the feeding and management should be strengthened to prevent the occurrence of this disease. |
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