The Epidemiological Investigation Of Bluetongue In Some Areas Of Xinjiang And Preliminary Separation Identification Of Strains

Bluetongue (BT) is transmitted by blood-feeding insects of bluetongue virus (BTV) and can causecattle,sheep,deer,camels and other ruminants high fever,mucosal edema,erosion and other symptoms,thedeath rate of20ï¼…to30ï¼…,some even up to90ï¼…,causing serious losses to the livestock production.2142serum samples were collected from Xinjiang8cities in2012and been serological testing for BTby agarose gel immunoassay(AGID). Result showed that the the positive rate in goat serum is range from0.51ï¼…to3.33ï¼…,highest of them is3.33ï¼…and the average is1.32ï¼…. All of bovine serum were negative.According to the results and history date,established monitoring fauna(10goat and10bovine)and collectedthe anticoagulants and serums every week for serological and pathogen detection. Competitive ELISA wasused to detect984serum samples from Yuli.209of them were positive,the positive rate was21.24ï¼….434goat serum positive rate was39.17ï¼…,550bovine serum positive rate was7.1ï¼…. The anticoagulants wereinoculated in chick. Collected liver grinding of the death click and infected C6/36cells first,and then toBHK21cells. Extracted RNA from cell SAP and confirmed the sample of bluetongue virus infection withthe nested PCR. Combined the epidemiological survey,we can got these:BT was widespread in Xinjiang.Both cattle and sheep can be infected. As for the infection rate,sheep is higher than bovine and the goat ishighest. It is the high incidence of BT infection in the5-10month of Culicoides activity. The Yuli ofBayinguoleng state is the BT natural epidemic foci. The positive rate of serum in south of Xinjiang,such asBayinguoleng,Aksu,Hami and Changji et al,are higher than that in the north of Xinjiang,such as Yili andAltay. The seropositivity rate of BT is that southern of Xinjiang higher than the northern.