| Equine rhinopneumonitis （ER） is a collective term for any one of several highly contagious, clinicaldisease entities of equids that may occur as a result of infection by either of herpesviruses, equidherpesvirus-1and equid herpesvirus-4Infection by either EHV-1or EHV-4character is by a primaryrespiratory tract disease of varying severity. EHV-4is causing respiratory tract disease primarily in horses;Infections by EHV-1in particular are capable of progression beyond the respiratory mucosa to cause themoreserious disease manifestations of abortion,perinatal foal death,or neurological dysfunction, whichbring a huge economic losses to the horse industry.In this study, by designing effective primers according to the conserved sequence of gB gene ofEHV-1and optimizing reaction system,a loop-mediated isothermal amplification (LAMP) approach wasestablished for detecting the EHV-1rapidly,and its specificity, sensitivety and reliability were evaluated.The results showed that the LAMP assay was determined to be10times more sensitive than a conventionalpolymerase chain reaction for detection of EHV-1. Furthermore, the higher specificity and reliability wereobserved. In conclusion, we established a rapid, sensitive and specific LAMP-based method for thedetection of EHV-1, which has important implications for the effective control of EHV-1, Through theEHV-1LAMP detection of54clinical samples; compared with test results of PCR methods have beenvalidated, coincidence rate is100%. The LAMP method in this test is rapid, specific, sensitive, easyoperation and low equipment requirement, have application prospect. |
