Expression H、F、M、P Protein Of Peste Des Petits Ruminants Virus Strain Tibet07in Insect Cells

In order to obtain purified peste des petits ruminants virus H, F, M and P protein, the H, F,M and P gene of peste des petits ruminants virus strain Tibet07was cloned into transfer vectorpFastBac/CT-TOPO individually. Each recombinant plasmid was then transformed intoDHl0Bac complement cells. The recombinant bacmid DNA was isolated and transfected intosf9insect cells to express each protein. The expressed protein was identified by usingSDS-PAGE, Western blot and indirect immunofluorescence assay. The resuLts showed thatthe recombinant H, F, M and P protein was successfuLly expressed in insect cells. This workprovides basis for the study of the biological function on peste des petits ruminants virusproteins.