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The Effect Of Different Surgery Methods Of Catheterization On Nutrient Absorption And Utilization In Goats

Posted on:2015-01-22Degree:MasterType:Thesis
Country:ChinaCandidate:Z Y LiuFull Text:PDF
GTID:2283330467968908Subject:Animal Nutrition and Feed Science
Abstract/Summary:PDF Full Text Request
This papper had investigated the effect of laparoscopic andtraditional laparotomy by which the goats were fitted with catheters onnu trient digestion and absorption、 immune stress and net free amino acid(FAA) fluxes across the mesenteric-drained viscera (MDV)、po rtal-drained viscera (PDV) an d liver in goats: This research wasdivided into three experiments:Exp1: Eight healthy young Qingjiang wether go ats with similar agewere divided into two groups according to the principle of similar weightand fitted with catheters in mesenteric vein an d portal vein bylaparoscopic and traditional laparotomy surgery methods respectively.The nutrient digestion and ab sorption of1~15d of postoperative weredetermined.The results sh owed that dry matter intake、the digestibility ofprotein and the utilization of nitrogen of laparoscopic group weresignificantly higher than that of laparotomy group(P<0.01) and thedigestibility of DM of laparoscopic group were remarkable higher thanthat of laparotomy gro up (P<0.05). The results of this experimentindicated that the effect laparoscopic surgery on nu trient digestion andabsorption in goats was smaller and goats recovered faster inlaparoscopic group.Exp2: Eight healthy young Macheng goats (ram) with similar agewere divided into two groups according to the principle of similar weightand fitted with catheters in mesenteric vein、 portal vein an d hepatic veinby laparoscopic an d traditional laparotomy surgery methods respectively.The serum levels of immune challenge index of preoperative andpo stoperative1、3、5d were determined. The results sh owed that theserum levels of IL-6、 TNF-a、 CRP rised first and following with declineof postoperative in two groups. The CRP levels of postoperative1d of laparoscopic group were sign ificantly lower than that of laparotomygroup(P<0.01) an d the IL-6、 CR P levels of postoperative3d oflaparoscopic group were remarkable lower than that of laparotomy group(P<0.05). The levels of TNF-a had no difference between two gro ups(P>0.05); The serum levels of IgA、 IgG、 IgA declined first andfollowingwith rise of posto perative in two gro ups. The levels of IgG ofpo stoperative1d of laparoscopic gro up were remarkable higher than thatof laparotomy group(P <0.05) an d The levels of IgA and IgM had nodifference between two groups(P>0.05). The results of this ex perimentindicated that the effect of laparoscopic surgery on immune ch allenge ingo ats was smaller and immune function of goats recovered faster inlaparoscopic group.Exp3: Eight healthy young Macheng goats(ram) with similar agewere divided into two groups according to the principle of similar weightand fitted with catheters in mesenteric vein、 portal vein an d hepatic veinby laparoscopic an d traditional laparotomy surgery methods respectively.Net free amino acid (FAA) fluxes across the MDV、 PDV an d liver ofpo stoperative5d were determined. The results showed that the netessential amino acid (EAA)、non-essential amino acid (NEAA) an d totalamino acid (TAA) fluxes across the MDV and liver and the EAA and TAAfluxes across the PDV had no difference between two groups (P>0.05).Net Pro fluxes across the MDV and Gly、 Ala and NEAA fluxes across thePDV were remarkable higher than that of laparotomy group (P <0.05).Theresults of this experiment indicated that the effect laparoscopic surgeryon the NEAA fluxes across the PDV of postoperative5d in goats wassmaller....
Keywords/Search Tags:laparoscopic, catheterization, goats, nutrients, immunestress, free amino acid
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