Functional Analysis Of TaSTKC8,TaNBS And TaRF2a Genes On Wheat Leaf Rust Resistance In TcLr19

Leaf rust, caused by Puccinia triticina, is one of the most destructive diseases of wheat. Dueto the physiological races specialization of leaf rust, some resistance genes may loss thoseresistance with the mutation of pathogenic race. Discovery and utilization of favorable genes inwild sourse, study the resistant mechanism and prolongation resistanct cultiver’s life become veryimportant task to reduce the damage caused by wheat leaf rust. A leaf rust resistance gene Lr19has been transferred from Agropyron elongatum and located on the long arm of wheatchromosome7D. It provides high resistance to all races of P. triticina currently found in Chinaand has great potential to be used for wheat production. The functional analysis of the resistancerelated genes TaSTKC8, TaNBS and TaRF2a cloned from TcLr19were carried out by usingBSMV-VIGS techinique and subcellular localization. The results have important implications forthe further study of the resistance mechanism to leaf rust. The main results were as follows:1. TaSTKC8and TaRF2a were located on wheat plasma membrane and nucleus bysubcellular localization. TaNBS was located on plasma membrane.2. Three genes were carried out for further functional analysis using BSMV-VIGS technique.The infection type of control wheat TcLr19was “0”, while wheat plants of silenced geneTaSTKC8showed infection type of “X”, so does TaRF2a, and wheat plants of silenced geneTaNBS showed infection type of “3”. Further histological observation indicated the same results.Plenty of mycelia were observed in the plant with silenced gene TaSTKC8, TaNBS or TaRF2a at120hpi. The results indicating that the products of all three genes are required for Lr19-mediatedresistance to09-12-284-1（THTS）.