Study On The Functions Of TaATG8Gene In The Process Of Plant Resisting Stresses

Autophagy is a degradation pathway for damaged organelles and long-life proteinwidely exists in eukaryotic cell,it plays a critical role in maintaining cell metabolicbalance,normal growth and development and environmental adaptation during the thestress condition such as hunger, oxidation,drought, salt, pathogen infection,or the processof cell differentiation, cell death and aging, etc. Previous work in this lab proved that twocore protein of wheat autophagy degradation pathway: ATG6and ATG8are involved in theprocess of wheat leaf rust resistance infection.On this basis, arabidopsis wild-type andAtatg8mutants are used as materials, the TaATG8overexprssion positive plants wereobtained by Agrobacterium Floral-dip method.Further, the role of wheat ATG8in abioticstress,(drought, salt, ABA, the lack of nutrition) and biotic stress (powdery mildewinfection) are clarified.Through biomass statistics,we know that TaATG8gene may playsome roles plants’ growth and development. The mainly results are as follows:1. The TaATG8gene was cloned from the wheat seedlings’ leaves.2.Through bioinformatics analysis, AtATG8and TaATG8amino acid sequence has highhomologous, similarity of84.03%. Moreover,autophagy body is labled by MDC after thearabidopsis is darked,the result turns out that TaATG8arabidopsis restored AtATG8function, and in the leaf senescence process by dark,AtATG8andTaATG8are both involvedin the formation of autophagy body, they have highly conserved function.3. The overexpression vector pSuper1300＋-TaATG8was constructed then transformedinto the Arabidopsis theliana by Agrobacterium Floral-dip method. Hence, the TaATG8overexprssion positive plants were obtained.4. Arabidopsis ATG8gene pure mutation are get through molecular verification, andthis mutation was transformed by Agrobacterium Floral-dip method,the TaATG8backmutation positive plants were obtained.5. Through biomass statistics,we know that siliques and seeds of arabidopsis are bothincreased as a result of TaATG8gene overexprssion, thus, TaATG8gene may beyield-related.6. Through the salt, drought and lacking of nutrition stress experiments, ATG8genemay be related to arabidopsis response of sugar deficiency, salt, drought, ABA andoxidative stress, these stress have great effect on the germination rate of seed; TaATG8andAtATG8gene play a very important role in arabidopsis thaliana seedlings resisting against various kinds of abiotic stress;ATG8gene plays an important role in the process of droughtand nutrient stress in grown-up arabidopsis thaliana;We confirmed that TaATG8gene mayplays an important role in plant resistance to abiotic stress.7. By infecting arabidopsis with powdery mildew we confirmed that TaATG8geneplays a positive regulation role in the process of arabidopsis defending against powderymildew.