Genetic Transformatiom Of Chi&Glu Gene And The Expression Of Two Members Of PGIP Gene Family Induced By SA&JA

In order to elucidate the function of chitinase and β-1,3-glucanase genes and providethe basis for its widespread use in disease resistance breeding, bivalent plant expressionvector of chitinase gene (Chi) and β-1,3-glucanase gene (Glu) was transformed into theleaves of tobacco‘NC89’ mediated by Agrobacterium tumerfaciens. With the material of’Qinguan’ and ’Hokuto’ apple leaves, the effect of SA&JA inducing on the expression ofPGIP gene family in apple leaves were studied. The main conclusions were as follows:1Bivalent plant expression vector containing both Chi and Glu was transformed intotobacco ‘NC89’ leaves mediated by Agrobacterium tumerfaciens.14kanamycin-resistantplants were acquired at100mg·L-1Kanamycin selection pressure, PCR detection resultsshown that Chi and Glu gene both positive in12resisntance plant, only Chi gene or Glupositive in1kanamycin-resistant plants, respectively. Target gene has stably inherited to theT1generation confirmed by PCR detection.2Transcriptional expression pattern of PGIP1and PGIP2gene from apple leaves PGIPgene family, which was induced by0.01mmol·L-1SA&0.1mmol·L-1JA, was different.The induced expression of PGIP gene family in leaves of ‘Qinguan’ is higher than that ofPGIP in leaves of ‘Hokuto’ treated by0.01mmol·L-1SA. After treated by0.1mmol·L-1JA,compared to the contorl, expression level of PGIP1and PGIP2gene in ‘Qinguan’ and PGIP1gene in ‘Hokuto’ was induced, but expression level of PGIP2gene in ‘Hokuto’ wassuppressed during48h; in addition, The transcription expression of PGIP1gene from appleleave in ‘Qinguan’ and ‘Hokuto’ was represented by single-peak curves.