Characteristcs Of The Transposition Activity Of Mariner-like Elements From Phyllostachys Pubescens(Edulis)

Transposon(Tn), also known as the transposable element (TE), skip factor, mobile gene, jumpinggene, is the basic unit exsiting in chromosomes which is moblie and able to autonomous replicate. Infact, Transposons are defined as DNA sequences that are able to move from one location to another inthe genome, or from one chromosome to another.In this study, two mariner-like transposons (Phmar1and Phmar2) with full-length transposasefrom Phyllostachys pubescens(edulis), and the nonautonomous partners (Phmar1NA and Phmar2NA)were fused to the vector pBIN m-gfp5-ER which contains green fluorescent protein gene, and then thecomponents were transferred into the Arabidopsis thaliana genome via Agrobacteria-mediatedtransformation. The T1of transgenic A. thaliana plants were screened and identified. By the methods ofFluorescence observation and PCR, transposition activities were detected in the transgenic plants withPhmar1and Phmar2but, by contrast,not in the ones with Phmar1NA and Phmar2. The results suggestthat the mariner-like elements Phmar1and Phmar2are active and able to have heterologoustranspositions in model plant A. thaliana.Detecting the transposon footprints and the reintegration sites of Phmar1and Phmar2in thetransgenic plants, both of the two mariner-like elements are tend to leave transposon footprints duringthe transposition activity, and are able to reinserte into another location of A. thaliana genome. By theanalysis of the sequences of transposon footprints with bioinformatics software, the mechanism ofexcision of Phmar1and Phmar2were found different，that means the characteristcs of the transpositionactivity of these two mariner-like elements are different to each other.In this study, the heterologous transposition in A. thaliana of mariner-like elements fromBambusoideae were demonstrated for the first time. And the characteristcs of the transposition activityof them were also analized successfully. These results would contribute for the next plan of hyperactivemutants from P. pubescens(edulis) mariner-like elements and producing a new genetic tool based onmariner-like elements available for plant gene tagging.