The Technical Research Of Hericium Erinaceus Polysaccharide Extraction

Hericium erinaceus is also called hericium，it belongs to Basidiomycotina, PolyporalesHydnaceae and Hericium, it is also a precious drug combination. Hericium erinaceuspolysaccharide is one of the main active component of hericium erinaceus, it has the functions ofregulating immune，anti-aging, anti-tumor, fall blood sugar, fall hematic fat, resisting mutation,radiation protection, etc. In this paper the writer uses the hericium erinaceus fermentation myceliaas raw materials to research on the extraction efficiency of key factors on the process of myceliumpolysaccharides and extracellular polysaccharide extraction to optimize the polysaccharideextraction process.1. In the process of previous studies and on my pilot study to determine the hericiumerinaceus submerged fermentation medium as the followings:1) Potato dextrose medium (PDA): potato20%, glucose2%，AGAR2%, water1000ml,121℃sterilization20min.2) Liquid seed culture medium: potato20%, glucose2%, Yeast extract0.1%, Potassiumdihydrogen phosphate0.2%, Magnesium sulfate0.1%, VB10.01%、pH6.5，121℃sterilization20min.3) basal culture medium: Soluble starch2.5%, yeast extract,2.5%, potassium dihydrogenphosphate0.1%, Magnesium sulfate0.1%, VB110mg/100mL、pH5.0，121℃sterilization20min.2. On the basis of determination of the optimal fermentation medium, we conduct the singlefactor experiments of different temperature, the initial pH, loaded liquid, inoculums and tablespeed. Through the liquid shake flask culture, we studied various factors influence on mycelialbiomass and extracellular polysaccharide. We determined the optimal conditions for hericiumerinaceus shake flask culture as:25℃、pH5.5，Shake bottled liquid volume100ml/250ml,inoculum size7.5ml, the table speed140r/min.3. In the process of extraction of hericium erinaceus fermentation myceliumpolysaccharide，We had a single factor experiment analysis of main factor which affect Hericiumerinaceus mycelia polysaccharide extraction yield, including leaching temperature, ratio ofmaterial to water, Leaching time, cocnentration factor, ethanol concentration, leaching times. Atthe same time, we worked out L9(34)orthogonal experiment. We ensured the optimal extractiontechnology for mycelium polysaccharide fermentation according to leaching temperature,Leaching time, concentration ratio and ethanol concentration, etc. Liquid-solid ratio of10:1,leaching temperature90℃,leaching time2.5h, Leaching times twice, enrichment ratio of4:1,final concentration of ethanol precipitation polysaccharide70%. After repeated washing sediment by anhydrous ethanol and Vacuum freeze drying to get the mycelium coarse polysaccharide.4. In the process of hericium erinaceus fermentation extracellular polysaccharide extraction,we Orthogonal design four factors of concentration multiple, concentration temperature,concentration of alcohol analysis and extracting liquid pH value. and we determined the optimumextraction technology of extracellular polysaccharides as followings:Remove centrifugal filtrate of mycelium of pH6.0,80℃concentrated to1/3of the originalvolume, After cooling to join precooling95%ethanol to make its final concentration of75%.Place4℃refrigerator, centrifugal precipitation collecting4500min, After repeated washingsediment by anhydrous ethanol and Vacuum freeze drying to get the mycelium coarsepolysaccharide.