The Metabolism And Residues Of Norfloxacin Formulation In Anguilla Japonica

2010, the Ministry of Agriculture announced more than100GB fishery formulation,because of its composition, shape, size and other aspects of the standard and rationality, toreplace the bulk drugs used in the past. Various fishery drugs preparations in the metabolicprocesses and the degree of utilization of aquatic animals has not yet been reported. This paperstudies the four norfloxacin (NFX) preparations in the Japanese eel (Anguilla japonica) in vivopharmacokinetics dynamics, bioavailability, tissue distribution and residue depletion lawprovides a theoretical basis for the development of rational drug program and the withdrawalperiod. The water temperature and middleweight of eel were (28±0.5)℃and (480±59) g,respectively. The dosage was equal to30mg NFX/kg body weight. The drug concentrations intissue were determined by Ultra high performance liquid chromatography-tandem massspectrometry instrument (UPLC-MS/MS).Following a single dosing oral of four NFX four formulations mixed feed in eel, Cmax wasall reached in3or4h, but was significant difference. Norfloxacin powder (NP) and norfloxacinberberine hydrochloride premixes (NB) were the highest to1.273and1.073mg·L-1, nicotinicacid of norfloxacin soluble powder (NN)0.616mg·L-1, lactate norfloxacin (NL) as low as0.944mg·L-1. The four formulations of the elimination half-life (T1/2) ascending respectively:15.27h(NP),24.89h (NB),25.89h (NL),33.04h (NN). Area under concentration time curve (AUC)of NN was14.18mg·L-1·h-1, for each formulation relative bioavailability greater degree ofdifference, NN: NP: NB: NL=100%:160%:158%:21%. of NP and NB strong efficacy, butshorter duration of action. NN and NL efficacy is weak, but the long duration of action.Absolute bioavailability of NL in different mode of administrationis was also supported byintravenous injection as a reference. Intramuscular injection and mixed feeding were38.62%and4.57%respectively. This shows that the mode of administration has a significant effect onthe absorption and utilization of drugs. Norfloxacin eel is widely distributed in vivo in14tissues can be detected, with higher levels of the intestine, stomach, gall bladder and liver, andshowed a different variation. Continuous feeding of Japanese eel3d norfloxacin powder mixedwith feed, norfloxacin was higher levels in muscle and kidney, the elimination half-life was4.8days in muscle and in the liver, kidney and plasma, respectively, for5.5d6.8d and7.5d. If50μg·kg-1is the highest residue limits, the withdrawal period of norfloxacin in the muscle, liver,kidney and plasma was18.62d,12.21d,27.44d and5.71d, respectively.