The Antimicrobial Active Ingredient In The Fermentation Product Of Streptomyces Albospinus (58#)

Actinomycetes is the main source of antibiotics, antibiotics and other substances in its secondary metabolites play an important role in the prevention and control of agricultural pests. Wuzhishan is one of the highest original tropical rainforest in the world, and the ecological function is complete and the actinomycetes resource is rich. Many actinomycetes strains possessing antimicrobial activities have been collected from its soil, and preliminary identifications have been made by the group of Professor Zeng Huicai. This study was designed to isolate the bioactive from one strain of the actinomyces.In this study, the fermentation broth of one actinomycete strain (Streptomyces albospinus58#) was extracted with ethanol, and the compounds from the extract were isolated by column chromatography, then the structures of all the compounds were elucidated by physicochemical and spectral data (UV, IR, MS, D NMR and D NMR). Nine compounds were isolated from the extract of the fermentation liquid of Stretomyces albospinus, and Seven known compounds were elucidated as4a,10β-dimethyldecahydronaphthalene-1α,3β,5α,7α-tetraol (1),2-(4-methoxyphenyl)-2-(propylamino)ethanol(2), cytoxazone(3), N-(2-Hydroxy-l-(4-methoxyphenyl)ethyl)acetamide (4), p-methylphenol (5), p-hydroxybenzoicacid (6), and/7-dimethoxy benzene (7).The anti-microbial activities of all the compounds were tested by paper disco diffusion method. Compounds1,3,4, and6showed anti-microbial activities against methicillin Resistant Staphylococcus aureus (MRSA), compounds8and9showed anti-microbial activities against Fusarium oxysporum f. sp. cubense race4, Fusarium oxysporum f.sp niveum, and Fusarium oxysporum f.sp vasinfectum. Among them, the anti-MRSA effects of compounds3,4, and6were reported for the first time.