Predictive Analysis Of Function On Circadian-Related Gene And Preliminary Study On Mechanism Of Rhythmic Expression Of Carbon Starved Anther(CSA) In Rice

In the whole growth process of plants are in a changing environment, such as temperature, light,air, and one of the most significant changes are light alternating, thecircadian rhythm. The plant will use the circadian clock to feel the circadian rhythm, and then adjust the floweringtime, photosynthesis, metabolism, growth, physiological processes such asthe regulation of plant seed. The regulation mechanism of biological clock further is limited within Arabidopsis, rice which is the main food crops in China, to understand themechanism of rice biological clock biological process, on the basis of plant science is not only beneficial to the understanding of the problem, for the improvement of Agronomic Traits in riceare also important significance. This study is mainly about the two aspects of rice circadian regulation mechanism:1) we used the bioinformatics to analyze the characteristics, biological function, using known circadian clock as bait, and co expression withbiological clock rhythm gene expression.Found that the biological clock rhythm of rice gene expression of circadian clock gene expression in Arabidopsis with same peak. At the same time, in network analysis the co expression was found that, biological clock genes in rice and interact to form a three co expression network subset, consistent with known Arabidopsis circadian clock model.Explanation of the interaction mechanism of the biological clock core oscillator is conservativein different species. But in the input channels and output pathway, as compared with Arabidopsis and rice for monocotyledonous environmental response, regulation of biological processes may have different mechanisms. For biological network analysis is the expression of the bait, found that changes in a day in the biological clock geneexpression preferentially occurs in the morning and afternoon and the same phase of thegene co expression form a subset of that rice biological clock gene through interaction and orderly, so that complex life process orderly. And to have the ability to predict future changes in the environment. The co expression network in the rhythm of the expression of GO genefunction analysis, found a number of biological processes may be involved in the regulation ofthe biological clock of rice, which can process and metabolic regulation of stress response process. The biological clock of main control to different stress reaction and metabolismgenes and different time, the biological clock can be speculated on stress response andmetabolism is regulated strictly in an orderly manner, at the same time also ensures that theenergy loss of plant in response to environmental changes in the process of consumption.2) we had isolated a photosensitive male sterile mutant csa(carbon starved anther), the expression pattern of CSA analysis showed that CSA has a circadian rhythm expressioncharacteristics of CSA promoter analysis revealed that the expression of CSA gene may bethe rhythm control factor, light response factor, hormone response factor of cis. When the rice seedlings treated by continuous light, rhythmic expression of CSA still exists, butweakened, said CSA is regulated by the biological clock, but may also be influenced by other factors. In a single optical processing, reduce the expression of CSA, at the same time in the light receptor mutants corresponding, increase the expression of CSA, indicating that CSA expression is regulated by light inhibition. The circadian clock genes, includinggenes of starch metabolism of sugar transport, CSA gene, gene co expression of quantitative analysis, found that the circadian clock may be involved in the regulation of sugar transportand rice starch synthesis by regulating the expression of CSA.The above results proved that the circadian clock may be involved in the regulation of ricemetabolism and stress response, and the CSA gene may be an important part of a circadian clock adjusts certain biological processes. According to the particularity of the csa mutant, we can also indirectly shows that the circadian clock plays an important role in the transformation of fertility in rice.