The Transcriptome Study Of Stipa Breviflora In Desert Steppe Under Simulated Warming

Stipa breviflora belong to grassy, Stipa spp. It is constructive species in desert grassland, high-quality forage, has high fodder and ecological value. This study using Illumina sequencing technology to transcriptome sequencing fruit vegetative stage Stipa breviflora by which continuous simulated warming since 2006 in desert steppe, and verified the RNA-seq results, screened Stipa breviflora reference gene under simulation warming.At last,combined with transcriptome data,using real-time PCR analysis cellulose synthesis-related genes.The results are as follows:1.Obtained totally 131779 transcripts,54075 Unigenes after homologous alignment.2.2.Obtained 3613 simulated warming-related genes of Stipa breviflora,there are 2696 regulated genes,and 917 down-regulated genes.In GO functional classification "metabolic process", "catalytic activity" and "binding" is the most enriched GO DEGs entries, respectively 1164 DEGs,1158 DEGs and 1132DEGs.In KEGG pathway annotations result, a total of 1791 DEGs were classify in 151 metabolic pathway.3. TLF is the most optimum reference gene under simulated warming.4. Simulated warming inhibiting the starch synthesis in Stipa breviflora post-fruiting vegetation stage, accelerate the sucrose decomposition process, and promote the synthesis of cellulose substrate accumulation of UDP-Glc.5.The cell cycle-related genes did not change significantly.6. Total cellulose content of Stipa breviflora leaves in different stages of growth were rise up under simulated warming(P< 0.05), the most increase happen in post-fruiting vegetation stage (8.56%), amounting to 50.56% of the total dry matter.This study built Stipa breviflora transcriptome information platform, to understand their transcriptome level changes response to global warming, systematic excavation its differentially expressed genes, combined with gene expression patterns to explore its growth and development, glucose metabolism and cellulose biosynthesis gene regulation mechanisms and modes.And established the most optimum reference geneunder simulated warming.Provide experimental evidence for the protection of the future of wild forage yield and quality, resource conservation and formulate of the future germplasm development strategy and other aspects.Meanwhile provide a reliable theoretical basis to prediction adapted mode and prospects for perennial grasses of this region,to develop ecological protection strategies and study on quality animal forage production.