| Through microbial enzymatic hydrolysis and fermentation of the poor fish meal into a higher nutritional value of products, in the domestic and international research less, especially at home still does not have relevant research. This study used bacillus, microzyme and Lactobacillus plantarum fermentation inferior strain three with fish meal, proteinase activity, acid soluble protein content, and the content of histamine content of Escherichia coli and Salmonella as index, effects of single strain fermentation and mixed culture fermentation of bionic inferior fishmeal nutritional value, determine the best fermentation process conditions of the single strains and mixed strains fermentation inferior biomimetic fish meal.First, Effects of different fermentation on nutritional value of inferior fish meal. The optimal fermentation conditions of different species of bacteria test. Bacillus subtilis inferior fishmeal combined conditions the best fermentation:aerobic fermentation, fermentation time 60H, fermentation temperature 30℃,3% inoculation quantity, the ratio of material to water 1:0.9; crude protein quantity under the condition of inferior in fish meal by fermentation fermentation increased prior to the 44.23% to 46.45%, acid soluble protein content increased from before fermentation 1.78% to 13.16%, neutral protease and alkaline protease activity increased from 6u/g to 385u/g before fermentation, histamine, Escherichia coli and Salmonella significantly reduced. Yeast fermentation of inferior fish meal combinations of conditions the optimal fermentation:aerobic fermentation, fermentation time 48h, fermentation temperature 30℃,2% inoculation quantity, the ratio of material to water 1:0.9; the content of crude protein in this condition in fish meal by fermentation of inferior improve before fermentation of 44.23% to 46.32%, increased from 1.78% to 9.45% before fermentation of acid soluble protein content, histamine, Escherichia coli and Salmonella were obviously decreased. Lactobacillus plantarum fermentation quality fishmeal combination of optimal fermentation:anaerobic fermentation, fermentation time 72h, temperature 37℃,2% inoculation quantity and ratio of material to water 1:1; the content of crude protein in this condition in fish meal by fermentation of inferior improve before fermentation of 44.23% to 46.63%, increased from 1.78% to 10.02% before fermentation of acid soluble protein content, lactic acid content reached 2.35%, histamine, Escherichia coli and Salmonella significantly reduced.Then, Effect of multi strain solid fermentation of inferior raw bionic fish meal nutrition value. The aerobic fermentation and anaerobic fermentation, bionic two stage fermentation, orthogonal test was carried out to optimize the fermentation conditions of inferior strains, optimum fermentation conditions of multi strain solid fermentation of inferior raw bionic fish meal, i.e. total fermentation time 96h,48h by Bacillus subtilis and yeast 30℃ aerobic fermentation, after 48h plantarum and rumen microbial anaerobic bio fermentation at 37℃; three kinds of bacteria in the fermentation of simultaneous inoculation, the total proportion of inoculation 6%, Bacillus subtilis, yeast and Lactobacillus inoculation were 2%,2%,2%; aerobic and anaerobic fermentation stage, the ratio of material to water is 1:0.9. Fermentation under these conditions, the fermentation quality fish meal in the protease activity was 489u/g, acid soluble protein content of up to 20.32%, histamine, Escherichia coli and Salmonella and other anti nutritional factor was decreased significantly, the content of crude protein increased from 44.23% to 48.12% before fermentation, lactic acid content reached 2.18%. |
