The Construction Of Stem Tip Culture System And A Study Of Efficient Cultivation Technology In Chufa

The materials of this experiment was the Chufa. This experiment mainly studied the construction of stem tip culture system and the influence of yield and quality of different seeding time and different irrigation cycle in Chufa. The main research results were as follows:(1) The tip culture system of Chufa was set up. The explant of the experiment was stem tip. The experiment screened the best medium of differentiation,proliferation and rooting medium through different ratio of hormone. The experimental results showed that: you can get a good disinfection effect by 70%alcohol immersed for 30 s and 0.1% mercuric chloride for 10 minutes. The best differentiation medium was MS+6-BA1.0mg/L+NAA0.3mg/L. The best proliferation medium was MS+6-BA1.0mg/L.The best rooting medium was 1/2MS+IBA0.1mg/L.(2) We found the best seeding time in Wuhan through the experiment.It’s essential to choose suitable sowing date as the seeding time was very long.The treatment of highest dry matter accumulation was the sowing time in March; The treatment of the highest yield was April and the area yield was 11643.81kg; The treatment of highest crude fat content was May. According to the experimental results we found that the best seeding time was May.(3) We found the best irrigation cycle through the experiment.When the irrigation cycle was one week, the dry matter accumulation and yield was the highest.The area yield was 8549.91 kg.The treatment of highest crude fat content was one week.The content of the crude fat was 24.22%. According to the experimental results we found that the best irrigation cycle was one week.