Water Hyacinth In High Value Application And The Control Of Ecological

Water hyacinth is a global harmful aquatic weed, as invasive alien specie, has been flooded in southern China waters. This paper combines ecological cycle concept, using biological treatment technology, water hyacinth systematically investigated, and the propagation of ampullaria gigas spix and earthworms use of water hyacinth investigated, purification earthworm lumbrokinase and antimicrobial peptides investigated. And strive to control water hyacinth, at the same time, make the water gourd get high-value use. Results will provide guidance for further experimental and industrial production.Growing conditions of water hyacinth and effects of purification quality are investigated. Water hyacinth cultivated in the optimal temperature of 30℃, the optimum pH of 6.5-7.5, earthworm point l-2%o. Purification of water hyacinth in water, nitrite first increased and then decreased trend. Ammonia is decline. Nitrate nitrogen content showed increasing first and then decreasing trend. And total nitrogen in water showed a declining trend. Content of total phosphorus and phosphorus content in water showed a declining trend. COD and chla in water showed a declining trend.The effects of ampullaria gigas spix use of water hyacinth are investigated. Water hyacinth was used by ampullaria gigas spix in the optimum pH of 6-8, the optimal temperature of 25-30℃, dissolved oxygen of 7.4-8.7 mg/L, water height of 25-35 cm. Under this condition, ampullaria gigas spix using water hyacinth, can gain weight rate most and feed coefficient lowest. After water hyacinth was used by ampullaria gigas spix, the proteins, polysaccharides, fats, crude ash, dry matter, showed a rising trend. This experiment through optimal rearing conditions, the feed coefficient of using water hyacinth was reduced, weight gain of ampullaria gigas spix was promoted.The effects of earthworm use of water hyacinth are investigated. Water hyacinth was used by earthworm in optimum pH of 7-7.5, optimal temperature of 20-25℃, optimum density of 10%, a height of 10-20 cm, the humidity of 70-75%. Under this condition, ampullaria using water hyacinth, can gain weight rate most and feed coefficient lowest. After water hyacinth was used by earthworm, the proteins, polysaccharides, fats, crude ash, dry matter, showed a rising trend. Through optimal rearing conditions, the feed coefficient of using water hyacinth was reduced, weight gain of earthworm was promoted.Using potable water hyacinth earthworms as raw materials, two lumbrokinase were purified and characterized. Two lumbrokinase with Mw of 30 kDa and 29 kDa, of single subunit, were purified to homogeneity respectively, by using phosphate buffer extraction, ammonium sulfate precipetation, ultrafiltration, DEAE Sepharose fast flow chromatography, Sephadex G-100 chromatography. LK1 and LK2 properties are similar. Vermis kinase optimum temperature is 50℃, the optimum pH is 7.5-8.0. Relatively stable around the pH is 5.0-10.0. Thermal stability range is under 50℃. Two of lumbrokinase were severely inhibited by PMSF.Using potable water hyacinth earthworms as raw material, one antimicrobial peptide from earthworm were purified and characterized, one antimicrobial peptide with Mw of 20 kDa, of single subunit, by using electric shock-induced, phosphate buffer extraction, ammonium sulfate precipetation, ultrafiltration, SP Sepharose fast flow chromatography, Sephadex G-75 chromatography. Earthworm antibacterial peptides activity on bacteria was optimal at pH 6.0-7.0 The antibacterial peptide was stable in pH 4.0-11.0 and under 70℃.