The Production Technology Research About The Comprehensive Development And Utilization Of Sophora Alopecuroides

Sophora alopecuroides found in the northwest region,which not only contains a variety of the alkaloid can be used medical and health care,also contains rich effective components such as protein, sugar, and flavonoids, can be used as livestock feed rich in high nutrition. But after livestock ate the alkaloids in sophora alopecuroides, can cause bromatoxism. This article went for research of extraction and purification of alkaloids in Sophora alopecuroides, and research of the residue of Sophora alopecuroides after fermentation, as a new type of recycled high protein livestock feed, the Sophora alopecuroides from waste into useful feed and provide reliable theory basis and experimental basis for industrialized production of sophora alopecuroides is the subject of trials.This paper mainly studied the following content:1. The qualitative and quantitative method of alkaloids in sophora alopecuroides. Qualitative determination of total alkaloid and alkaloid monomer mainly by thin layer chromatography. Expansion agent reespectively for qualitative determination of total alkaloids, Sophocarpidine, Oxymatrine and Cytisine of thin layer chromatography were hloroform-methanol-ammonia (7:3:0.5),N-hexane-ethanol-ammonia(7:3:0.1),Chloroform-n-b utyl alcoho-ammonia(6:4:0.1) and ethyl acetate-methanol-ammonia(8:2:0.05). The quantitative determination of total alkaloids were determined by ultraviolet spectrophotometry and compared with the sophocarpidine as control. The quantitative determination of alkaloid monomer were determined by high performance liquid chromatography (HPLC) method.Quantitative determination conditions of matrine with Inertsil NH2, the wavelength is 220nm, mobile phase is:Acetonitrile-alcohol-3% of phosphoric acid water(75:15:10, V/V/V), Quantitative determination conditions of Oxymatrine and Cytisine with Inertsil C18, detection wavelength are 210nm and 310nm, mobile phase are:Methanol-acetonitrile-water-phosphoric acid(10:30:65:0.05, V/eV/V/V) and Acetonitrile-0.02 ammonium acetate-triethylamine, Quantitative determination of temperature are room temperature,the flow rate are all 1 mL/min, the injection volume are all 20 uL.2.The study on production process of The extraction of alkaloids from the sophora alopecuroides. Verify and determined the optimum extraction process conditions of the extraction of total alkaloid in sophora alopecuroides by single factor experiment and orthogonal experiment. The optimum experiment conditions are:the size of Sophora alopecuroides is 20-40 meshes, extraction solvent forsulfuric acid water of pH0.8, ratio of solid to liquid was 1:20, leaching temperature is 27-37℃, the best time of leaching for 14 h, extraction times are 3 times.3.The study on Alkaloids in sophora alopecuroides purification techno logy. The experiments including static experiments and dynamic experiments, The results showed that the optimal conditions were:static experiments select the best resin as D001, the optimum pH was 3.5, concentration was 7.5 mg/mL, best eluent was 65% ethanol elution of pH10 ammonia; The dynamic experiment determined that the best selection of the sample rate of the resin is 5.5 mL/min, eluent is 4 times ammonia 65% ethanol of pH10.4.The study of Sophora alopecuroides monomer separation technology. By crystallization and recrystallization, Separated matrine monomer that purity of 97%;Through the acid-organic solvent extraction, by silica gel column separation repeatedly, obtain the 96% purity of cytisine monomer crystals; By using oxidant, hydrogen peroxide oxidation oxidate matrine from matrine to oxymatrine,got the 95% oxymatrine purity monomer, conversion rate reached more than 90%.5. After extraction of alkaloids of sophora alopecuroides, residue after fermentation, obtained the high protein organic feed. In the fermentation process of fermentation temperature, determined the optimum process conditions of fermentation by fermentation time, initial moisture content and soluble solids content of research; By comparison adding culture to not add provides residue of sophora alopecuroides, optimized by adding yeast, fermentation feed is rich in protein, polysaccharide nutrients, can be used as a good feed instead of other optimal feed. The best fermentation conditions for temperature is35℃, time is 5 days, The initial water content is the percent of 60.