Development And Utilization Of EST-SSR Molecular Markers In Three Turfgrasses

At present, molecular marker of turfgrass is less. Molecular biology research and application is restricted in turfgrasses, three main turfgrasses tall fescue, bluegrass and clover were selected as materials. EST-SSR molecular markers were developed by bioinformatics and commonality analysis. EST-SSR markers were applied to the analysis of genetic diversity. The main results were as follows:732 pairs of EST-SSR primers from wheat, barely, maize, sorghum and rice five crops were selected to analyze the transferbility to three major turfgrasses, 406 pairs(55.46%) of primers had effective amplification bands in tall fescue;354 pairs(48.36%)of primers had effective amplification bands in clover. 457 pairs(62.43%) of primers had effective amplification bands in Bluegrass.According to the results of EST-SSR information analysis, 108 pairs of primers were designed and synthesized. 92 pairs of primers on tall fescue could amplify stable and clear bands; 93 pairs of marker based on clover were synthesized, 91 pairs of primers could amplify clear and stable bands.81 pairs of primers were choosed for the analysis of genetic diversity in 12 species of tall fescue, 79 pairs(97.53%) showed polymorphism; clustering analysis indicated that the twelve cultivars were clustered into five groups based on 0.81 of GS value; 107 pairs of primers(97.20%) were used for genetic analysis of five clover cultivars, red bean grass and clover.104 pairs of primers showed polymorphism, The analysis of genetic diversity on seven materials, dividing into two genotypes on 0.51 of GS value; 43 pairs of effective amplification primers were used for genetic analysis in 19 species of Bluegrass, between them 42 pairs(97.67%) showed polymorphism, Clustering analysis indicated that the nineteen cultivars were clustered into five groups based on 0.58 of GS.