Monitoring Of The Airborne Microorganism In Swine Houses

In order to evaluate the hygiene quality of different swine houses, air samples from five swine farms was collected using ANDERSEN-6 and the AGI-30(All Glass Impinger, AGI), and microbial concentration and air constituents of the breeding environment were measured.The study consists of three Parts:Part l : The concentrations of swine aibronre microbialUsing ANDERSEN-6 sample collector, air flow rate was 28.3 L/min, with 5% male sheep blood mediumfor sampling, the collector Settings in piggery central, such as height is 50-80 cm is placed higher than pig of nasal cavity, drive time according to different health conditions in 1 ~ 5min.The results showed that swine airborne aerobic content ranged from 0.466×104CFU/m3 to2.585 × 104CFU/m3 air,airborne anaerobic content ranged from 0.06 × 104CFU/m3 to 2.025 ×104CFU/m3 air,airborne fungal content ranged from 0.265 × 103CFU/m3 to 1.49 × 103CFU/m3 air,airborne gram-negative bacteria content ranged from 0.0141 × 103CFU/m3 to 0.84 ×103CFU/m3 air,On average only between 0.30 and 3.2% of the total number of culturable aerobic bacteria were identified as gram-negative bacteria.Part 2 : The concentrations of swine aibronre endotoxinInternational standards AGI- 30 collector put on the middle of the pigpen, with no pyrogen of 50 mL of water for the sampling medium, sampling time and space airflow rate was 12.5 L/min,drive time for 30 min.Collect samples of endotoxin content is obtained with the aid of LAL experiments.According to the sampling time and the air velocity calculating outgassing endotoxin content(EU/m3 air). The results showed thatthe airborne endotoxin content ranged from 0.114×103EU/m3 to 0.533×103EU/m3 air。Part 3: The concentration and composition of airborne garm-negative bacetria,and its aerodynamic diameterFirstly, the preliminary separation of gram-negative bacteria by gram’s staining;Second,gram-negative bacteria by 0-F test and oxidase test into enterobacteriaceae and the fermentation of gram-negative bacteria, respectively, with a API-20 E and API-20 NE for identifying.In the airborne gram-negative bacterial flora the following bacterial families dominated:theEnterobacteriaceae, the Pseudomonadaceae and Morexella.Within the family of the Enterobacteriaceae the species Escherichia coli a, Enterobacter agglomerans and Klebsiella were predominant.Steno trophomonas m altoph ilia is primary in airborne genus Pseudomonas;only lacuna Moraxella detected in each pig farm environment.In addition, Acinetobacter and Rhizobium were only isolated from farm C and D.There are 58% of aerobic bacteria, 48% of negative bacteria distribution on the collector layer 1, 2(particle diameter of 4.7 ~ 7 μm or higher),they access to people and animals of nasal airway, can cause upper respiratory tract infection;22%of aerobic bacteria, 39% of negative bacteria distributed on the collector layer 3, 4(2.1 ~ 4.7μm),they can enter the people and animals trachea, bronchi or bronchioles;And 22% of aerobic bacteria,13% of negative bacteria distribution on 5, 6 layer(0.65 ~ 2.1 μm), they tend to exist in the form of single cell, can pass in and out of bronchioles and alveolar, people and animals when inhaling too much and animal decreased body resistance, easy to cause the lower respiratory tract infections the survey found, it is distributed in 5, 6, Andersen collector level negative have clumps of enterobacteriaceae, kill bacteria Pasteur, lacuna mora pseudomonas bacteria and the stench.The results of this study will provide more information about microbial content and composition of the breeding environment and lay the foundation for environmental quality assessment, which is significant and instructive for environment hygiene control.