The Relationship Between PBG And Garlic Green

Garlic for liliaceae allium plants, the main edible part is the garlic bulbs, nutritious, high medicinal value. In addition to the table, garlic are often processed into mashed garlic, garlic juice, dehydrated garlic slice, garlic powder and other products, but often appear in the process of processing green growing phenomenon, the serious influence the quality of products. \ "Laba garlic and garlic green is\" the main color material, and after hundreds of years of traditional diet custom is verified its security, has the qualities of a development of natural pigment, additives, can be used as important application in food, pharmaceutical and other industries, the development potential is tremendous. Study the garlic green pigment biosynthetic pathway to explain garlic green growing mechanism, control of garlic products processing phenomenon of green in the process of change and development of natural pigment, promote the development of garlic industry is of great significance. This test by garlic bulbs as raw materials, from a central part of the plant physiology metabolism PBG of the Krebs cycle metabolites, the relationship between the studies with the green garlic, probe into its to the role of allicin by what way and make the green garlic, garlic green change mechanism and its control in the production of green provide new theoretical basis. The main results were as follows:1. The PBG and enzyme in garlic green effectExperiment to optimize the method of garlic extract PBG, cloves of garlic in PBG extraction yield can reach 0.106 ‰, and the garlic PBG content in green change process and the corresponding degree of garlic green change tracking measurement. Green in the process of change, the PBG waste samples of the green change effect is better. On the other hand, the PBG consumption of small samples, produce green effect is weak. Further validation of PBG in green, garlic and its consumption and garlic, green is associated with the degree of change.Further experiments, explores the enzyme in the important position between the PBG with garlic and green. In this experiment the experiment method of the design optimization of the green system of garlic consumed after enzyme method, the green before the change of garlic enzyme treatment, experiments prove the existence of the enzyme reaction with allicin is able to make the PBG and produce green. To further clarify the mechanism of garlic green variablestep PBG need through enzyme function into pigment precursor containing tetrapyrrole structure, which in turn involved in generating in the process of pigment.2. The different solvent system with garlic extract PBG, garlic, green variable relationship is exploredExperiment by using a variety of different polarity solvent to extract of garlic green becomes effective components, explore the solvent environment effect on green variable screening of effective components and its influence on the variation in content of PBG.Through various analyses of experimental phenomena of groups of samples, the experiment concluded PBG need and the role of a through enzyme produced and is soluble in alcohol and insoluble in ether material response, then further to the role of allicin produce green effect. At the same time, speculated that there is another kind of soluble in water and alcohol solution,but insoluble in ester substances in garlic green element generated within the two steps of before. Among them, alcohol phase system the most conducive to garlic, green change effects,but the most convenient for garlic, green, garlic and 60 to 80% of the alcohol solvent system green becomes the most obvious effect. In alcohol system at the same time there are 2 kinds of red pigment can produce, with green change its maximum absorption wavelength of 430 nm and 490 nm, respectively. In addition, for different pH worth garlic alcohol solvent system was studied, proved overly acidic environment destroyed the PBG structure, affected the formation of pigment precursor, which in turn affect the green, and blue garlic element in a basic environment is not stable, easy to form yellow pigment.