Effect Of Rhizobium And Molybdenum On Nutrition Uptake And Yield Of Soybean

In modern agriculture, the over-dose of fertilizer used has led to environmental pollution, soil degradation and other issues, so how to supply nutrition for crops reasonably and scientifically has been a research focus today. The symbiotic of rhizobium and legume could supply partly nitrogen for the growth and development of legume. And many elements affect the efficiency of nitrogen fixation of the symbiosis. At present the research of effect of rhizobium on legume is relatively common, but the yield-increasing effect is unstable. So how to improve or stabilize the application effect of rhizobium still need further research. Molybdenum play a very important role in nitrogen metabolic process of leguminous crops, and it will produce certain effect on efficiency of nitrogen fixation. In order to further clear the effect of the soybean inoculating rhizobium and fertilizing different concentrations of molybdenum, field experiments were conducted to study the effect of rhizobium and molybdenum on growth, nutrition uptake and yield of soybean in 2013 and 2014. The results showed:(1) Inoculating rhizobium only didn’t increase the biomass of soybean, which has no significant difference with non-inoculated and non-molybdenum treatment(CK1). The biomass of soybean in seedling and flowering stages was not increased by inoculating rhizobium and combined molybdenum I, but it was improved in podding, seed-filling and maturing stages. The biomass was respectively 21.05%, 9.16% and 10.27% greater than that of only inoculating rhizobium treatment(R) in 2013, and was respectively 5.06%, 11.92% and 11.95% greater in 2014 in podding, seed-filling and maturing stages. The biomass of inoculating rhizobium and combined molybdenum II treatment(R+Mo2) was 11.51% and 10.90% lower than that of inoculating rhizobium and combined molybdenum I treatment(R+Mo1), and that of only fertilizing molybdenum I treatments(Mo) was 3.33% and 5.01% lower than that of inoculating rhizobium and combined molybdenum I treatment(R+Mo1) in seed-filling and maturing stages in 2013 and 2014. Besides, the growth rate of soybean inoculated rhizobium and combined molybdenum I was 40.35% and 2.33% greater than that of only inoculating rhizobium treatment(R) from flowering to podding in 2013 and 2014.(2) Inoculating rhizobium and combined molybdenum I improved the N content of soybean root in seedling stage, and the N content of soybean stem and leaf in flowering and podding stages. The N content of root, stem and leaf were respectively 5.07%- 28.08%, 2.18%- 19.56% and 2.44%- 44.77% greater than that of only inoculating rhizobium treatment(R). And there was no significant difference between inoculating rhizobium and combined molybdenum II treatment(R+Mo2) and only inoculating rhizobium treatment(R) and between only fertilizing molybdenum I treatments(Mo) and only inoculating rhizobium treatment(R).(3) The N accumulation rate and N accumulation of soybean inoculated rhizobium only were not increased. While the N accumulation rate of soybean increased 6.40%- 52.64% by inoculating rhizobium and combined molybdenum I from seedling to flowering and from flowering to podding stages. And the N accumulation of inoculating rhizobium and combined molybdenum I treatment(R+Mo1) was increased by 2.34%- 32.41% when compared to that of only inoculating rhizobium treatment(R), and it was increased by 10.05%- 32.25% when compared to that of non-inoculated and non-molybdenum treatment(CK1) from flowering to maturing stages, and it was higher than that of inoculating rhizobium and combined molybdenum II treatment(R+Mo2). Besides, the P and K accumulation were also increased by inoculating rhizobium and combined 1% molybdenum.(4) The pods per plant and seeds per plant of soybean were improved by inoculating rhizobium and combined molybdenum I, they were respectively increased by 45.58% and 28.44% in 2013, and 52.96% and 57.23% in 2014 compared to that of only rhizobium inoculated treatment(R). Hence, the yield of soybean inoculated rhizobium and combined 1% molybdenum treatment increased respectively 20.46% and 9.63% in 2013 and 2014, and the yield increased margins were greater than inoculating rhizobium and combined molybdenum II(R+Mo2) and only fertilizing molybdenum I treatments(Mo).