Premarily Mapping QTL Underlying Growth Period Configure In Soybean Based On Four-way Recombinant Inbred Lines Population Accross Different Environment

For four-way recombinant inbred lines population, there are four alms in one locus, which come from four parents（kengfeng14, kengfeng15, heinong48, kenfeng19）. They contained 160 recombinant inbred lines as materials.In this study, The study of present research is to develop new methods for scgeregating analysis on major gene for FW-PIL,which would be used to predict the existence of major gene and soybean growth period QTL mapping and breeding.Thought scgeregating analysis on major gene for FW-PIL, these results have been proved:VE-R1 : there are several major genes in KS environment and there is no major gene in the other environment;R1-R3 : there is no major gene in the two environments of HS1 and HS2 amd there are several major genes in KS environment;R3-R6 : there is only one major gene in the two environments of HS1 and HS2 amd there are nol major gene in KS environment;R6-R8: here is no major gene in the three environments of KS, HS1 and HS2;R1-R8: there is only one major gene in the two environments of HS1 and HS2 amd there is no major gene in KS environment;VE-R8: there is only one major gene in the three environments.We used single marker analysis method and SSR discriminate genotype of grows period. The SF-ANOVA identified 52 markers as potentially associated with QTLs for VE-R1 flower at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased R1-R3 at QTLs identified on B2, A1, B1, C2, D1 a, D2, H, I, J, L, M, N. The beneficial genotype contained Sat₂64（A3A3）, BARCSOYSSR₁0₁444（A3A3）,ARCSOYSSR₁8₁813（A3A3）, Sat₁94（A3A3）å'Œ BARCSOYSSR₀8₀966（A3A3）. But Satt665 has been detected in three places（HS and KS）. Satt245, Satt002, BARCSOYSSR₁0₀066, Satt281 has been detected in two places（HS1 and HS2）. The SF-ANOVA identified 25 markers as potentially associated with QTLs for R3-R6 at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased R6-R8 at QTLs identified on D1 b, C2, G, L, N, K, E, D2, H, A2, O, J, M. The beneficial genotype contained BARCSOYSSR₀2₀607（A2A2）, Satt688（A1A1）, Satt346（A3A3）, Satt125（A2A2）, Satt557（A1A1）, Satt349（A1A1）, Satt288（A2A2）, BARCSOYSSR₀9₀849（A2A2）å'Œ Sat₀92（A1A1）. But Satt557 has been detected in three places（HS and KS）. Satt125 has been detected in two places（HS1 and HS2）. The SF-ANOVA identified 60 markers as potentially associated with QTLs for Pod—seed filling at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased Pod—seed filling at QTLs identified on A1, B1, B2, C2, D1 b, D2, E, F, H, I, K, M, N, O. The beneficial genotype contained BARCSOYSSR₁9₀356（ A4A4）,Sat₁95（A4A4）, BARCSOYSSR₀2₀607（A1A1）, BARCSOYSSR₁9₀451（A4A4）, BARCSOYSSR₁9₀466（A4A4）, Satt398（A2A2）, Satt577（A3A3）, Satt125（A2A2）, Satt002（A4A4）, Sat₄05（A3A3）, Satt354（A1A1） Satt284（A4A4）, BARCSOYSSR₀6₀011（A3A3）. But Satt125 has been detected in three places（HS and KS）. The SF-ANOVA identified 88 markers as potentially associated with QTLs for seed R6-R8 at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased seed filling--maturity at QTLs identified on 18 linkage groups. The beneficial genotype contained Satt273（A1A1）, Satt193（A3A3）. Sat₂64, Satt577, Satt345, BARCSOYSSR₁8₀105 has been detected in two places（HS1 and KS）. The SF-ANOVA identified 19 markers as potentially associated with QTLs for VE-R8 at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased First flower--maturity at QTLs identified on C2, D2, E, O, D1 b, L, F, B2, N. The beneficial genotype contained Satt577, Satt273, Satt458, BARCSOYSSR₁0₀066.Satt281. Satt557 has been detected in two places（HS2 and KS）. The SF-ANOVA identified 17 markers as potentially associated with QTLs for full period at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased full period at QTLs identified on A2, C1, C2, E, G. The beneficial genotype contained Satt557（A1A1）, Sat₃51（A1A1）.Sat₁25（A1A1）. But Satt307 has been detected in two places（HS2 and KS）. Satt199, Satt557, Satt125 has been detected in three places（HS and KS）.