The Effect Of Replacing FeSO₄ With Iron Glycine Chelate In The Equal Fe Level In Diets On The Broiler Chickens

Iron is an essential mineral for animal development and function. A study was carried out to evaluate the effect of replacing Fe SO4 with Iron Glycine Chelate(Fe-Gly) in the equal Fe level in diets on the broiler chickens. The main objectives were to investigate the effects of replacing Fe SO4 with Fe-Gly on the broiler chickens and to provide reference for the application of Fe-Gly in broiler production.A total of 600 1-day-old Arbor Acres commercial broilers with a BW of 45±1 g were used in a 21-d experiment. The broilers were randomly allotted to 6 dietary treatments with 5 replicate pens and 20 birds per pen. Treatments consisted of: Control group: 100 mg Fe from Fe SO4 /kg diet; Experiment group 1: 80 mg Fe from Fe SO4+20 mg Fe from Fe-Gly /kg diet; Experiment group 2: 60 mg Fe from Fe SO4+40 mg Fe from Fe-Gly /kg diet; Experiment group 3: 40 mg Fe from Fe SO4+60 mg Fe from Fe-Gly /kg diet; Experiment group 4: 20 mg Fe from Fe SO4+80 mg Fe from Fe-Gly /kg diet and Experiment group 5: 100 mg Fe from Fe-Gly /kg diet. After a 21-feeding period, the growth performance, immune organ index, blood biochemical parameters, iron concentration of tissues, antioxidant enzyme activities, and related gene m RNA expression in broiler chickens were measured.The results showed that replacing Fe SO4 with Fe-Gly in the diets cannot significantly improved the growth performance and immune organ index(P > 0.05). But it significantly improved the iron concentration of tissues and the blood biochemical parameter(P < 0.05). Xanthione Oxidase activity in blood serum had no significantly difference between all treatments at 21 th day except for experiment group 5(P > 0.05). In addition, Catalase activity in blood serum and Liver Cu/Zn superoxide dismutase activity in liver were increased with the increasing of the replacement level of Fe-Gly(P < 0.05). The hepcidin mRNA expression was increased with the increase of replacement ratio(P < 0.05). The divalent metal transporter 1(DMT1) m RNA expression was significantly decreased with the increase of replacement ratio(P < 0.05).But for all of the above indicators, the observed values of experiment group 3, 4 and 5 had no significantly difference(P > 0.05).In summary, the results of the experiment indieated that there was feasible in corn-soybean diets for broilers of 1-3 weeks-old substituting ferrous glycinate for the equal Fe level of ferrous sulfate. And the least substitution ratio for low feeding cost and beneficial effect on the broiler was 60%.