| Plant-height, ear-height, stem-diameter and tassel-branched-numbers are the most important agronomic traits in maize, is closely related with the yield of maize, close-planting resistance, lodging resistance and ecological adaptability. Studying and exploring gene positions which can control the plant height, ear height, stem diameter and tassel branched numbers of maize, legislating a scientific breeding programs with a definite object, and improving type traits of plant reasonably, has great practical significance on breeding of high-yield, density-tolerant, lodging-resistant maize.In this study, the parents with significantly different plant traits of maize inbred lines were selected to prepare the hybridized combination; hybrid F2 generation plants as the mapping population were selected to construct the SSR molecular marked genetic linkage map.192 F2 plant traits data were combined with molecular marker linkage map information, and the threshold value of LOD was determined by 1000 sampling, The QTLs of plant-height, ear-height, stem-diameter and tassel-branched-numbers were detected using composite interval mapping method in maize whole genome level. Main results were showed as below:1 Constructing the SSR molecular marked mapIn this study, the maize inbred line JS037 (female parent) and JS133 (male parent), which were collected from Anhui Longping high-tech seeds co.,ltd, were selected as the parent material; and 192 F2 plants were obtained as the mapping population to screen of molecular markers, which were related to the plant traits of plant-height, ear-height, stem-diameter and tassel-branched-numbers. Among the polymorphism between the parents screened through 300 pairs of SSR primers,121 sites were meet the requirements and used to analysis the genetic linkage relationships by Joinmap3.0 software. And a maize SSR molecular marker genetic linkage map with 127 sites,10 linkage groups, total length 1148.4 cM, and average distance 9.11 cM between different markers was obtained. According to the maize genome sequence information,10 linkage groups were located in the corresponding chromosome.2 The positioning analysis of QTLs of four plant type traits of maizeFour plant-high related QTLs were detected in 10 chromosomes. Whereas the qPH-1 is located on chromosome 2, additive effect value is-8.59, the phenotype contribution rate was 8.4%; two plant-high related QTLs were detected in chromosomes 3 (qPH-2, qPH-3), additive effect values were -6.84,-13.57, and the contribution rate was 7.6% and 15.4% separately; qPH-4 is located on chromosome 7, additive effect value is-8.68, which can explain 6.9% phenotypic variation. One ear-height related QTL (qEH-1) were detected in chromosome 7, the additive effect value is-8.43, which can explain 19.8% phenotypic variation. Three stem-diameter related QTLs (qSD-1, qSD-2 and qSD-3) were localized in chromosome 3,10 and 10, the additive effect values were-1.66,-1.53 and-1.16, respectively, which can explain 11.1%,15.4% and 7.4% of phenotypic variation. Three tassel-branch-numbers related QTL (qTBN-1, qTBN-2. and qTBN-3) are located at chromosomes 4,8 and 9, the additive effect values were-1.19,1.27 and-2.18, the contribution rates was 18.3%,24.7% and 33.7% respectively. In the chromosome 3, among the interval of umc1307-umcl399, The plant-height, ear-height and stem-diameter of QTLs (qPH-3, qEH-1 and qSD-1) were detected at the same time. |
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