Fungistasic And Growth Promoting Activity Of Marine Bacillus Atrains And Amplifird About The Control Gene Fragments

615 strains isolated from different marinie environments were screened for the antimicrobial avtivity.2 strains of Bacillus with pesticide potential were identified. The growth characteristics and relevant control genes of 2 strains were determined.The important biocontrol genes were amplifled; The control effect of tomato leaf mould and rape sclerotinia for the strains were investigated. All the experimental results as follows:(1) Gene fragments related with antifungi activity of ituC, fenD, srfAA, srfAB, bioA and bmyB were found in 2 strains. The minimum inhibitory concentration (MIC) of 2 strains to A.solani were 1 μl/ml (C12-6) and 2μl/ml (Z10-3)and antimicrobial spectrum on Botrytis cinerea、Alternaria solani、Fusarium graminearum, Cladosporium fulvum、Sclerotinia sclerotirum、Fusarium oxysporum、Phytophthora capsici、Pyricularia grisea of the Z10-3strain from 74.1% to 85.4%, the C12-6 strain was from 63.1% to 83%. The control effects with the strain Z10-3 of tomato leaf mould and rape sclerotinia were 62.1%～63.6%, and the strain C12-6 were 59.1%～ 65.4%.(2) Gene clusters related with growth promoting were detected in 2 strains.Gene fragments related with indole acetic acids(IAA) of ysnE and yhcX were assayed in 2 strains and phy gene of phytase was found in Z10-3 strain.In 2 strains, gene fragments of alsS, alsD and alsR were found, which controlled synthesis of2,3-butanedio. The functions of 2 strains with phosphorus solubilization, potassium release, nitrogen fixation and IAA production were tested. C12-6 and Z10-3 strains have dissolved nitrogen fixation and phosphorus activity, and Z10-3 strain has the activity of releasing. C12-6 and Z10-3 strains can produce IAA and siderophore-producing activity were 28.9% and 68.7%.The results showed that 2 strains could colonize in rhizosphere soil, roots, stems and leaves of the tomato. The Z10-3 root colonization in simulate saline soil was preferable with the maximum amount of colonization were 60×106cfu/gand decreased 18×106cfu/g 15days later; The C12-6 root colonization in simulate saline soil was preferable with the maximum amount of colonization were 60×106cfu/g and decreased 15×106cfu/g 15days later. As for tomatos treated with 2 strains,the effect of tomato growth-promoting were significant by 2 strains, improved tomato fresh weight, plant height and root length., its chlorophyll content incressed 53.4%(C12-6r) and 57.4%(Z10-3r) at the Salt stress.(4) The Z10-3 strain was identified as Bacillus amyloliquefaciens by 16SrDNA, gyrB sequence, physiological and biochemical; The C12-6 was identified as Bacillus sp.