MicroRNA Profile Analysis Of Epithelioma Papulosum Cyprini Cell Line Before And After Spring Viremia Of Carp Virus Infection

Micro RNA(mi RNA) plays significant roles in regulating almost all of the biological processes in eukaryotes, such as apoptosis, cell differentiation and proliferation, and DNA repair. many researches show that mi RNAs are associated with cellular changes following viral infection. Spring viremia of carp virus(SVCV) is the pathogen of Spring viremia of carp(SVC), which results in heavy losses in the cultured common carp(Cyprinus carpio) industry in many countries. To study the involvement of mi RNAs during SVCV infection, we adopted the Solexa sequencing technology to sequence small RNA libraries from the Epithelioma papulosum cyprini(EPC) cell line before and after SVCV infection.In this study, using high-throughput sequencing technology, all the small RNAs were annotated into different categories. A total of 161 conserved and 26 novel mi RNAs were identified.The expression patterns of these mi RNAs were compared between the uninfected(control library, M) and SVCV-infected(infection library, E) libraries. In SVCV-infected cells 10 mi RNAs were significantly up-regulated and 4 down-regulated.To verify the Solexa sequencing results, the expression patterns of some randomly selected mi RNAs were validated by q RT-PCR.The targets of the significantly differentially expressed mi RNAs were then predicted, and the mi RNAs that could directly target the SVCV genome were also predicted.A preliminary screening of mi RNAs related to viral replication was studied by transfecting mi RNA mimics or inhibitor, TCID50 assay and q RT-PCR.To the best of our knowledge, this study presents the first mi RNA profiling assessment in association with fish rhabdovirus infection, and the data presented lay a foundation for further investigations to determine the roles of mi RNAs in regulating the molecular mechanism during SVCV infection.