Studies On The Endophytic Fungus From Ipomoea Pes-caprae And Its Secondary Metabolites

Ipomoea pes-caprae is perennial herbs of genus Ipomoea batatas. It has long been used in medicine to cure many diseases including rheumatism, bloated, inflammation, etc. As medicinal plants Ipomoea pes-caprae contain resin glycoside and some scholars have been isolated resin glycoside from the Ipomoea pes-caprae. Study of plant is to develop bioactive chemical composition. Large scale use of the plant origin with biological activity requires many dredging plant resources, which is not conducive to protecting the ecological environment. To study endophytic fungi provides a good prospect for obtaining abundant natural medicine resources. Endophytic fungi are microbial resource with high diversity with a variety of metabolites. In this paper, our purpose is to screen out endophytic fungi which can produce resin glycosides from Ipomoea pes-caprae and to study it’s the metabolites.About 120 endophytic fungi were isolated from Ipomoea pes-caprae. The metabolites were screened out by TLC, MJ-8 was considered to produce resin glycoside. The fungus was identified as a Pestalotiopsis based on it’s morphological features. The fungus was identified as a new strain throμgh taxonomic identification.Statistical experiment designs were used to optimize the fermentation medium for maximum resin glycosides production based on Czapek’s medium. Alpha-D-Glucopyranose, and ammonia sulfate were selected as the suitable carbon sources and nitrogen sources via one-factor-at-a time design. According to the optimization results the best fermentation is 16 days. We get the fermentation fluid of MJ-8 from the fermentor. We studied the metabolites with column chromatography and HPLC. And 10 compounds were isolated and purified. These compounds were identified by means of 1DNMR and 2D spectroscopic techniques. The compound A6 was identified as a resin glycosides. The compound was identified as butanol-lactose glucoside.