Isolation And Identification Of A Bacteria Strain With Bio-control Function And Preparation Of Its Bio-organic Fertilizers

The rapid development of livestock and poultry breeding industry has brought great waste disposal pressure, and development of sustainable agricultural prompts to change the current situation of relying too much on chemical fertilizers, pesticides. Biological organic fertilizer prepared by using bio-control bacteria plant pathogen antagonist function of and poultry waste can realize the recycle of animal waste and can provides excellent production material for sustainable agriculture. For this purpose, some explorations and study were conducted in this paper and the main results are as follows:1. A strain named SCI with strong antagonistic effect to the anthrax bacteria was screened based a series of flat confrontation tests and the co-culture experiment between SC1 and anthrax bacteria in soil with the help of DGGE technique.2. The strain SC1 was identified as Paenibacillus polymyxa according to the sequencing result of itsl6SrDNA PCR amplification and by Blast analysis.3. By the means of the FISH technology in combination with counting methods, a series of simulation and large-scale composting experiments with different amount of inoculums, different inoculating times and different nutrition additives were conducted to determine the proper production process of biological organic fertilizer so as to improve the number of bio-control function bacteria SC1 in the biological organic fertilizer product. It was found that the composting process could be promoted by inoculating 1% of SCI fermentation broth to compost material during its high temperature stage and the number of SCI in biological organic fertilizer product could be significantly increased by inoculating 1% of SCI fermentation broth and adding 2% of monosodium glutamate wastewater as nutrient additive during cooling stage of composting.