Isolation Of Investigation Of Escherichia Coli In Sick And Dead Duck Embryos And Investigation Of Bacterial Resistance To Antibiotics

Escherichia Coli can not only cause the death of duck embryos during the hatching of eggs, but induce infection of ducks in large proportion, which has generated great damage on duck industry. Due to the excessive abuse of antibiotic, Escherichia Coli has possessed extensive and severe resistance to sorts of antibiotics. To investigate the resistance of Escherichia Coli(E. coli) to antibiotics in Tai’an, the study has isolated the pathogenic bacteria from 120 sick or dead duck embryos collected from a duck farm in Tai’an and tested their resistance to antibiotic.1. 36 E. Coli isolates of were identified by bacterial culture, biochemistry test, and the characteristic all fits for the features of E. Coli. The 16 S rDNA of positive bacteria were detected by PCR, the result showed that 36 isolates of E. Coli was identified by PCR, and the homology was 99% by the alignment from Genbank.2. The resistance of isolated E. Coli to antibiotics was analyzed by kirby-baner method. The result suggested that 36 isolates of E. Coli presented resistance to different antibiotics to different extend. The resistance rates are streptomycin(77.8%), gentamicin(61.1%), kanamycin(55.6%), spectinomycin(61.1%), cephazolin(44.4%), norfloxacin(50%), cefixime(38.9%), cefoperazone(44.4%), cefotaxime(55.6%), amoxicillin(77.8%), ofloxacin(72.2%), iprofloxacin(83.3%), a-mikacin(55.6%), polymyxin(945%), tetracycline(83.3%). The results of the study have a guiding significance on the clinical prevention and treatment of duck E. Coli in Tai’an area.