Detection Of Hsp90α In Plasma Of Chickens With Tumors In Different Process

Viral tumors are more common in chickens, resulting in decreased performance and increased mortality of chicken. Bring huge economic losses to the poultry industry. With the development of molecular mechanisms of tumor and the anti-tumor vaccine discovery, the most important problem is clean chicken virus,and we should solved it, with the understanding of the mechanism of tumor depth, the important role of heat shock proteins in tumorigenesis is gradually revealed. Heat shock protein 90α(Hsp90α) is a chaperone protein that can assist protein folding and maintain a variety of intracellular signal transduction,thereby, promoting cell survival and growth. In 2009, Zhang Luo, Tsinghua University, his team announced the world’s first human tumor cell-specific regulation mechanisms secreted Hsp90α, and at the first time revealed differences of the Hsp90α intracellular and extracellular molecules Hsp90α the difference. The team is further evidence of secreted Hsp90α can promote tumor invasion and metastasis, and its content and the degree of malignancy in blood positive correlation. After a number of clinical trials have demonstrated successful Hsp90αhuman lung cancer tumor markers that can be used to monitor and evaluate the efficacy of the patient’s condition. However, the relationship between tumor disease and poultry Hsp90α has no clear conclusion. In this paper, the main research work in poultry occurred viral tumor disease(avian leukosis and Marek’s disease), the blood levels and changes in Hsp90α; and acute transformed ALV for quail.This test will be chicken acute fibrosarcoma slurry in two ways abdominal and subcutaneous inoculation of 1-day-old Hy-Line Brown chickens attack drug for three weeks to take anticoagulants, 2 000 r / min 4℃ centrifugation 2min, suction the upper plasma,Whichever 100μL( remaining plasma stored at-80 ℃) was inoculated into already adherent CEF 96-well cell culture plate, after 2h changes,and keep cell culture maintenance medium for 7 days, the supernatant by ALV-p27 antigen assay kit. This test will be grinding fluid while acute fibrosarcoma; the remaining plasma is used to detect the plasma content Hsp90αby the method of pathological HE staining were observed and histopathological study of tumor tissue in the process of development of viremia, in chicks and adult quail take subcutaneous and intraperitoneal inoculation of virus attack way to study quail fibrosarcomaacute viral susceptibility. MDV GX0101 strains isolated from chickens at home and abroad to integration has reticuloendothelial hyperplasia virus long terminal repeat of the first strains of recombinant wild strains. The test of Marek’s disease tumor models constructed by artificially inoculated intraperitoneally GX0101 strains for three weeks to take anticoagulants, 2 000 r /min 4 ℃ centrifugation 2min, take the upper plasma CEF cells were inoculated, after 5 days,we begin to detect cell supernatant virus infection, the remaining plasma viremia development process for the detection of plasma Hsp90α content and methods of pathology by HE staining were observed and histopathological study of tumor tissue. The results showed that Hsp90α average content of avian acute leukemia tumor group was higher than the helper virus group and the control group; Hsp90α average content of poultry Marek’s disease virus attack group was higher than the vaccine group and control group.Described by chicken blood tumor virus after infection Hsp90α content has increased to some extent, suggesting that the virus-induced increased expression of stress induce Hsp90α,enhance cell viability in adverse environments. This conclusion is late to establish the correlation between tumor in poultry and pave the wayfor Hsp90α content, help researchers applied on poultry to poultry Hsp90α hematologic tumor disease research tissues targeting. In the study of acute susceptibility fibrosarcoma of quail, whether it is a day or60-day-old, chicks and adult quail intraperitoneal or subcutaneous inoculation, p27 detection,dot blot tests were negative, did not detect the presence of the virus for necropsy view, no grossly visible tumor nodules characteristic of tumor lesions. Show quail fibrosarcoma of acute non- susceptible virus, the body does not produce acute fibrosarcoma or quail can not produce viremia after infection. Mechanism of acute defective virus on quail further need more research.