| China is one of the major drought countries in the world, arid and semi-aridregions accounted for47%of the land area and51%of the total cultivated area[1]. Sum of crop losses caused by drought stress is higher than that caused by other stress[2]. Therefore China had focused on the drought resistance. The potato is a typicaltemperate crop and is very sensitive to water deficit. Drought will affect the normalgrowth and late production formation of potato and becomes the limiting factor in thedevelopment of the potato industry. Therefore we should comprehensively andsystematically research the drought damage and drought resistance mechanisms ofpotato and take effective measures to improve the drought resistance of potato toensure high and stable yield of potato.To better understand the dynamics of transcription drought stress, potato"Longshu3" seedlings are used as test materials in this study and Solexahigh-throughput sequencing technology is used to build a database of potato seedlingstranscripts and conduct correlation analysis. qRT-PCR technology is used to detectexpression patterns of potato drought-related genes to reveal the drought tolerantmechanism of potato in the molecular level. This study achieved the following results.1. Analysis of86,965,482RNA-Seq reads permitted the detection andquantification of expression levels of7,284genes at transcriptional levels, amongthem,6,754genes were enriched in draught-treated leaves while6,419in control. Weidentified842drought-responsive up-regulated and494down-regulated candidategenes with significantly differentially expression under continued drought stresstreatments.2. Differently expressed genes were mostly enriched in89gene categories and21KEGG pathways. These results provide a broad spectrum of candidate genes forscreening potato drought-related genes, which is essential for analying the molecularmechanism of potato in response to drought stresses.3.22differentially expressed genes with log2(fold change)≥11obtained bySolexa high-throughput sequencing were selected including6transcription factors,4genes related to proline metabolism,4genes related to antioxidant defense system,4genes related to biosynthesis of flavonoids and4genes related to plant hormonesignal transduction. The expression differences of the22genes in potato leaves undertreatment by20%PEG6000for0h,1h,6h and12h were determined using qRT-PCR method. The result showed that the22gene expressions were consistentwith the results from Solexa high-throughput sequencing, and there existed5expression profiles, including up-up-up, down-down-down, up-down-up,up-down-down, up-up-down regulation. The result could provide foundation for theresearch on drought-related gene function and drought resistance mechanism ofpotato. |
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