A Preliminary Study On The Effect Of Water Stress On Characteristics Of Physiological And Biochemical Properties Of Sugarcane Treated With Ethephon And1-methylcyclopropene In Sugarcane Seedling

Our study take the ROC22as test materials, to Spraying200mg/L ethephon+1-MCP and300mg/L ethephon+1-MCP then with a concentration of1mg/L1-MCP in a closed continuousprocess16h under different drought stress. By measuring the relative degree of drought stresscontrol soil moisture soil. We measured the sugarcane seedling leaf peroxidase (POD),superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), proline (Pro), solublesugar content, soluble protein and chlorophyll content of physiological and biochemical studythe effects of ethylene and1-MCP on the drought resistance of sugarcane, research results are asfollows:1, The results of this study showed that with enhanced drought stress, MDA content insugarcane leaves increased significantly, separate spraying1-MCP, spraying200mg/L ethylene+1-MCP compared with the control can maintain the content of MDA at a low level, help toreduce membrane lipid peroxidation of cell membranes to maintain the integrity of cellmembranes, resulting in a positive impact on sugarcane response when subjected to droughtstress, while spraying300mg/L ethylene+1-MCP treatment only in mild drought would benefitlower MDA content, and reaches moderate or severe drought, will exacerbate the accumulationof MDA, and secondary treatment effect is more pronounced.2, The findings of this study, subject to drought stress, the separate spraying1-MCP,spraying200mg/L ethylene+1-MCP can prevent a substantial increase in the accumulation ofsoluble sugar content and to some extent, delaying the cane leaves degradation rate of solubleproteins, thereby increasing intracellular osmotic adjustment ability to withstand drought stressand spraying200mg/L ethephon+1-MCP spraying alone is better than1-MCP. The spraying300mg/L ethephon+1-MCP produce only mild positive impact of drought, when the arid reaches ofmoderate to severe, will significantly increase the accumulation of soluble sugar, soluble proteindegradation accelerated, resulting in a negative impact affect the effectiveness of the moreobvious secondary treatment.3, Subject to drought stress alone after spraying1-MCP, spraying200mg/L ethylene +1-MCP Pro content than the control treatment, and spraying200mg/L ethylene+1-MCPtreatment effect of reducing the content of its Pro more significant, probably due to be sprayedafter treatment, can reduce the extent of the persecution of sugarcane leaves a lot to reduce theaccumulation of leaves Pro. The spraying300mg/L ethephon and1-MCP treated Pro content wasdecreased first rising trend in the severe drought stress will accelerate the accumulation of Pro’s.Secondary treatment effect is more obvious than the first treatment.4, In the continuing droughtconditions, the soluble protein content of four treatments sugarcane leaves increased firstly andthen decreased. On the4thday of the moderate drought periods the soluble protein of spraying200mg/L ethephon+1-MCP treatment (4.271mg/g) and spraying300mg/L ethephon+1-MCPtreatment (4.269mg/g) was significantly higher than only spraying1-MCP treatment (3.973mg/g),when on the5thday to7thday up to the time of moderate to severe drought stress, spraying300mg/L ethylene+1-MCP treatment soluble protein began to reduce and eventually up to2.198mg/g, lower than the control (2.380mg/g), spraying1-MCP treatment(2.684mg/g), andspraying200mg/L ethephon+1-MCP treatment (3.164mg/g). The twice treatment of solubleprotein content of a trend consistent with the once treatment.4, When under drought stress, sugarcane leaf POD activity since the3rdday increasedsignificantly and showed an increasing trend. The sugarcane leaves POD activity of spraying1-MCP treatment and spraying200mg/L ethephon+1-MCP treatment has been higher than thecontrol from the2ndday, and spraying200mg/L ethephon+1-MCP treatment the largest increasePOD activity was15.67%, significantly higher than spraying1-MCP treatment12.09%. Whilespraying300mg/L ethephon+1-MCP treatment only in mild drought stress, sugarcane leaf PODactivity higher than spraying1-MCP and spraying200mg/L ethephon+1-MCP treatment.However, in moderate and severe drought stress during drought stress, sugarcane leaves its PODactivity gradually decreased until the7thday, the sugarcane leaf POD activity from524.00(U/g min FW) fell488.00(U/g min FW) and has been lower than the control514.00(U/g min FW), POD activity of twice treatment is consistent with the once treatmenttrend. Also the SOD and CAT variation trend had the same trend with POD activity.5, Under drought conditions, the chlorophyll content of the four treatments coerced showinga decreasing trend, gradually lower than the normal four process water supply. Among them, spraying1-MCP treatment (2.132mg g-1FW) and spraying200mg/L ethephon+1-MCPtreatment (2.155mg g-1FW) from the3rdday of chlorophyll content was higher than control(2.091mg g-1FW), after also basically is slightly higher than control. The chlorophyll content ofspraying300mg/L ethephon+1-MCP treatment on the3rdday was2.186mg g-1FW greater thanspraying200mg/L ethephon+1-MCP treatment (2.155mg g-1FW) and separate spraying1-MCP(2.132mg g-1FW) and control (2.091mg g-1FW), and with increased drought stress reducedchlorophyll content increased to the7thday reached1.583mg g-1FW, below the spraying200mg/L ethephon+1-MCP treatment (1.797mg g-1FW), but still higher than separate sprayingof1-MCP treatment (1.556mg g-1FW) and control (1.465mg g-1FW).In summary, under drought stress, separate spraying of1-MCP treatment, spraying200mg/L and300mg/L two concentrations of ethylene and1-MCP treatment seedling sugarcane,through the determination of sugarcane seedling physiological and biochemical indicators,results indicates that under drought stress, separate spraying of1-MCP and spraying200mg/Lethephon+1-MCP could increase drought resistance, and spraying200mg/L ethephon+1-MCPtreatment, to improve drought resistance effect is obvious better than separate spraying of1-MCP; spraying300mg/L ethephon+1-MCP treatment, only in mild drought stress will helpimprove the drought resistance of sugarcane, but when moderate and severe drought stress,300mg/L ethephon was not conducive to improving drought resistance of sugarcane.