| Lanzhou lily is placed in family Liliaceae and is a variety which comes fromLilium davidii, It is a kind of local famous vegetable varieties in Gansu province andhas high value in edibleness and medicine. due to lack of an in-depthand systemsstudy of its genetic background and phylogenetic relationship, There are limitation forLanzhou lily management and breeding,so we analysied Genetic diversity amongeight populations of Lanzhou Lily by using ISSR molecular markers, and constructedits core collection basis on molecular data. The main results were as follows.1For genetic diversity analysis,A total of59distinct bands were detected with8ISSR primers among8populations,51bands were polymorphic，Percentage ofpolymorphic loci can reach86.3%,The variation ranged from4to9bands withan average of7.4.2On the species level，the observed number of alleles(Na) and effective numberof alleles(Ne)of Lilium davidi was1.8644and1.3464，respectively．Nei’s genediVersity(h)and Shannon’s information index(I) of Lilium davidi at species level was0.2163and0.3413.3On the population level,the population of Kangjiashan Cun had the highestpercentage of polymorphic loci(77.97％),while the population of Xiguoyuan townwas the lowest(50.85％).The population of Kangjiashan Cun had thehighest(Ne=1.3681),and the population of Xiguoyuan town was the lowest with theeffective number of alleles(Ne=1.2259).The population of Kangjiashan Cun had thehighest Nei’s gene diversity(h=0.227)),while the population of Xiguoyuan town wasthe lowest(h=0.1454). The Shannon diversity index of Kangjiashan Cun populationwas the highest(I=0.3509),while the Shannon diversity index of Xiguoyuan townpopulation was the lowest(I=0.2288)．4According to analyzed by POPGENE，the genetic differentiation coefficient(Gst)among populations was0.1582． the genetic differentiation coefficient amongpopulations((Hsp-Hpop)/Hsp)calculated by the Shannon diversity index was0.1582.These data demonstrated that the genetic variation of within-population accounts for the most of genetic differentiation among Lanzhou lily populations．Theindex of gene flow is3.6452indicate there are gene flow among differentpopulations.5The dendrograms of genetic relationships among populations were constructedbased on Nei’s(1978)unbiased genetic distance by means of UPGMAshowed that the8Lilium Davidi populations could be divided into2big groups, the population ofXiguoyuan town was included in group1.the population of Weiling Xiang,ShangyingXiang,Huangyu Xiang,Agan town,Jiangjiashan Cun,Doujiashan Cun,KangjiashanCun were in group2.6Based on data of ISSR analysis, Nei&Li, SM and Jaccard genetic distances forUPGMA cluster to core collection by multiple clusteringwere studied, SM geneticdistances using stepwise used finally to construct core coilection from175LiliumDavid jgermplasm,and fmally,37accessions were chosen as core collection．The corecollection hold21.14％samples of initial collection, and the reserved rate of numberof polymorphie loci，percentage of polymorphic loci, Observed number of alleles,Effective number of alleles,Nei’s gene diversity and Shannon’s information indexwere96.08%,96.59%,98.18%,101.93%,105.27%,103.62%,respectively.There wereno Positive difference between Nei’s gene diversity,Shannon’s information ofPrimary core colleetion and total colleetio according to t-test, Those result indicatethat the construced Primary core colleetion could represent genetic diversity of thetotal collection. |
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