Comparative Proteomic Analysis Of Potato Leaf Between Diploid And Tetraploid

Solanum tuberosum L, a polyploidy plant species, there are a great number of germplasm ofhaploid and polyploidy in the nature, solanum tuberosumsubsp.tubero, highly heterozygousautotetraploid crop as well as asexual reproduction. Diploid species including cultivated speciesand wild species, as a pivotal gene pool resisted to disease, pest, toleranced to low temperature,heat and reducing sugar content was lower. Solanum tuberosum, which have some characters,such as narrow genetic background, poor resistance gene pool, closed genetic relationship. Inorder to illustrate the change and mechanism of resistance, quality, development, metabolismamong diploid and tetraploid. The agronomic trait, physiology characteristics, leaf proteomicbetween diploid and tetraploid were used to clarify the difference.In this study, diploid species cv（.S.goniocalyx）pa mpuna,（S.stenotonum）morada7aruna,（S.goniocalyx）7HIRI0006.05, tetraploid species cv. Atlantic, QS2（Qingshu2）, GN2（Gannongshu2）used as materials. The characters of floral phase, including the color of stem,leaf, corolla, main stem number, the length of stolon, the appearance time of flower bud andstolon, the plant height, stem diameter was recoded, the content of chlorophyll, soluble sugar wasexamined. The characters of tuber, including tuber shape, the color of skin and fresh, skin type,eye depth, tuber uniformity, tuber number of every plant was recoded. The content of starch,reducing sugar and dry matter was determined. The comparative proteomics of leaf protein fromdiploids and tetraploids were analysised. The results were as following:The characters of morphology of potato diploid and tetraploid were recorded. The stolonlength of morada7aruna were increased by2.8and2.6times longer than pa mpuna and7HIRI000605, respectively. Pa mpuna has lower plant height. The stem diameter of morada7aruna were increased by1.1and1.3times as higher than pa mpuna,7HIRI0006.05, respectively.The content of chlorophyll in7HIRI0006.05were increased by1.6、1.9times higher than pampuna, morada7aruna. The contents of soluble sugar in morada7aruna were increased by1.2and1.1times higher than pa mpuna and7HIRI0006.05, respectively. The tetraploids havedifferent characters. The stolon length of Atlantic were increased by4.4and1.4times longer thanQS2and GN2. The plant height in GN2were increased by1.2and1.3times higher than Atlanticand QS2, respectively. The stem diameter of GN2were increased by1.2and1.5times higher than Atlantic and QS2. The content of chlorophyll in GN2were increased by1.3and1.2timeshigher than Atlantic and QS2. The content of soluble sugar in Atlantic were increased by1.2and1.1times higher than QS2and GN2, respectively.The tuber characters of diploid and tetraploid were as follows. The characters of morada7aruna tuber are round, white skin, and deeper eyes. The tuber numbers of morada7aruna wereincreased by1.9and1.5times than pa mpuna and7HIRI0006.05, respectively. The starchcontent of pa mpuna were increased by13.7and1.9times higher than morada7aruna and7HIRI0006.05; the dry matter content of morada7aruna were increased by1.3、1.2times higherthan pa mpuna and7HIRI0006.05, respectively. The contents of reducing sugar were increasedby0.9and1.5times than pa mpuna and morada7aruna, respectively. The tetraploid varietieshave un-unifomity characters. The tuber number of QS2were increased by1.0and1.5times thanAtlantic and GN2, respectively. The starch content of Atlantic were increased by5.4and2.3times than QS2and GN2, respectively. The reducing sugar of GN2were increased by1.2and1.1times higher than QS2, respectively.The differentially expressed protein spots were detected in diploid and tetraploid. Theprotein spots were1000±40,680±25,915±10,750±20,1200±16,1200±45in Atlantic,QS2, GN2, pa mpuna, morada7aruna and7HIRI0006.05, respectively. Forty-seven differentiallyexpressed protein were detected in diploid and tetraploid. Eleven proteins were detected indiploid species. Fourteen proteins were detected in tetraploid species. One protein was detectedin GN2, pa mpuna and7HIRI0006.05. Ten proteins were identified in GN2.Forty–five proteins were identified by mass spectrometry. The function of proteins includedmetabolism, photosynthesis, stress and defense, protein synthesis and other functions. Thedomain resistance proteins of diploid varieties included coproporphyrinogen-III oxidase, glucanendo-1,3-beta-glucosidase, peroxidase. The domain resistance proteins of tetraploid includedheme-binding protein and peroxidase. More relative photosynthetic protein including carbonicanhydrase, ribulosebisphosphate carboxylase, ribulose-1,5-bisphosphate carboxylase/oxygenaselarge subunit were identified in tetraploid compared to diploid.