The Cry1C~*Transgenic Rice’s Cultivation And The Epistatic Interaction Analysis Of Heading Date QTLs In Rice

Rice is one of the major food crops in the world. To cultivate high quality of Early-maturing rice is an important way to improve grain production.To cultivate high quality of Early-maturing rice, I have made the following work:In the aspect of cultivating high quality rice, I using genetic engineering technology, make rice transgenic technology combine molecular assisted breeding markers to cultivate the insect-resistant rice, so as to achieve the aim of high yield and high quality. Stem borers and Cnaphalocrocis medinalis of lepidoptera insect moth caused serious rice production is one of the main pests. Because some δ-endotoxins of Bacillus thuringiensis (hereinafter referred to as Br) gene encoding have highly specific insecticidal activity to lepidoptera pests. I optimize Bt gene codon to get the cry1C*, which have a screening marker bar gene, implanted in high-yield, high-quality, high rice varieties (Sheng13, Sheng17. Luxiang NO.1) by carcinoma agrobacterium mediated. After detection of Basta daubed, laboratory PCR and phenotype in the field experiment, oBtained four Sheng17varieties of plants successfully.In the aspect of cultivating early rice, according to study some rice heading date QTLs and rice field investigation data, to analysis the control gene and affect of gene. Aggregating these genes by microsatellite markers, according with field investigation to analysis the interaction relationship between them.Heading date is one of important agronomic traits in rice, and decides its adaptation to diverse cultivation areas and cropping seasons. Through the control of the rice heading stage to avoid the borers season to achieve the purpose of production.In this study, we use43single segment substitution lines with the same genetic background to research rice heading date. The following conclusions have get out of the analysis of the heading date QTL polymerization. Hd7gene on NY001have epistasis contrast with Qhd8b gene on NY006; Qhd8b gene on NY006have epistatic interaction contrast with QHd-3gene on NY010; QHd-3gene on NY010have epistasis contrast with Ehd-1gene on NY032; QHd4gene of NY024inhibits the expression of Hd6c gene of NY020.