Ft Gene Spatially And Temporally Expression In Mulberry Varieties

Induce flowering plants not only about the continuation of the species, and is closelyrelated to human agricultural production and economic life. FT (FLOWERING LOCUS T)and homologous genes integrated in the process of flowering induced signal more regulatorypathway plays a key role in signaling molecules, from the signal receptor-leaf meristemconducted to induce the plant to reproductive growth transformation, but also FT gene may beassociated with perennial seasonal dormancy related. Thus, the expression of FT genesaffected by day length, circadian rhythm and other environmental signal. However, the roleof flower induction and regulation of other aspects of sleep FT homologous genes in woodyperennials Mulberryhas not been reported. This research for the promotion of geneticbreeding process and improve the yield of mulberry fruit, regulate sleep and otherenvironmental adaptability have important theoretical and practical value. In this experiment,total mulberry RNA extraction methods optimized,investigate the expression of FT to clearrelationship between the expression of FT and flower regulation. FT expression of mulberryvarieties from different geographical origins were investigated, analyzed the relationshipbetween the relevant regulatory FT gene in Mulberry Sleep laid a theoretical foundation forthe application of mulberry FT gene.This test is divided into three parts:1. Mulberry physiological indexes under drought stress researchTo know mulberry drought damage physical and analyzes the formation mechanism ofdrought resistance, use of5%PEG on new varieties of mulberry Ichinose simulate milddrought stress treatment for14days,from14day to20day rehydration. Determination of theimpact of drought on mulberry leaf relative water content, relative conductivity, proline andMDA content. The results show that the relative water content will continue to decrease underdrought stress.the relative conductivity,proline content and MDA levels are sustained increaseunder drought stress. After rehydration these four physiological indicators will tend to restorethe levels before the drought stress, but will not fully recover, indicating that there may bedroughts Mulberry memory.2. The comparison and improvement of Mulberry total RNA extracted from two kind ofTrizol and FT gene Real-time quantitative PCR analysis RNA extraction effect evaluation and analysis, screen the extraction method is suitablefor mulberry. According to the procedure specification, and OD260/280yield of RNA weredetermined by electrophoresis of extracted RNA were compared. OD260/280of RNAextraction byInvitrogen Trizol was suitable than RNA extraction by Tiangen Trizol,electrophoretic bands neat, indicating Invitrogen Trizol RNA is more suitable for theextraction of mulberry leaves. Simultaneously comparison isopropanol precipitation wasfound isopropanol precipitation time to appropriate increase to improve the purity of RNA.This test is optimized mulberry RNA extraction provided the basic data.FT gene expression is strictly controlled by environmental signals role, in order tounderstanding FT gene expression pattern mulberry in different months and different lightingconditions. The FT gene annual expression of six varieties were detected, the results showedgradual increase from April to June, FT gene expression si the highest at July,from August toNovember expression was gradually reduced. FT gene expression of New Ichinose and itsmutagenic strains triploid305Mulberry analysis throughout the day, at July of the highestexpression of the full-year and in the long sunshine sunny conditions at zero slightlydownward to3:00,6:00to15:00showing an increasing trend,15:00expression reaches itspeak,18:00to21:00expression was gradually reduced;whereas in the case of cloudy/rain,FT gene from zero to9:00expression was gradually reduced,then the expression of FTremained at a steady level without no significant fluctuations.It can be speculated that longhours of sunshine and high temperatures can make the FT gene expression upregulate,FTgene expression also persistent low activity in the absence of direct light conditions.FTexpression of different geographical origins of mulberry varieties and day length wereanalyzed,with significant inter-species differences in the expression of FT in different time.New Ichinose FT expression in rapid in the growth period, FT expression levels of otherspecies is significantly less than the level of expression of the new Ichinose;no significantdifferences between the various species at May,June,September and Octomber; at July NewIchinose has the highest expression, White Lotus has the lowest FT gene expression,othervarieties were no significant differences;707in August has the highest FT gene expression,FT gene expression of Liaoning and New Ichinose followed by707, the remaining differencewas not significant; at November707and White Lotus the expression level was significantlylower than Liaoning, Ukraine, New Ichinose and Wubao mulberry. So presumablyindependent regulatory pathways in different species will significantly affect the expressionof FT.3. Drought resistance gene PMSR cloning and expression Through homologous comparison cloning cloned PMSR gene fragment of expression,through sequence alignment, can prove that it belongs to mulberry polymorphic armour sulfursulfoxide reductase, successfully obtained the expression of gene fragment. Throughcomparative analysis grey value of PMSR gene and Actin gene amplification bands,PMSR inmulberry leaves were significantly increased relative expression genes after drought treatment9days. This trend of gene expression of drought stress and contrast is consistent withprevious laboratory protein two-dimensional electrophoresis analysis results, the expressionlevels of both protein and RNA levels rise after drought stress.