Preliminary Studies On Population And Bioactivity Of Educable Actinomycetes From Hyperhaline Extreme Environment In Xinjiang

Objective:To study the population and bioactivity of actinomycetes from soil in hyperhaline region of Xingjang, China and accumulate microbial resources. Methods: Using two isolation methods, four different kinds of cultural media,123isolates were purified from twenty e soil samples in salt field of Fuhai county of Altay and Lopnor area.100among123strains were cultured and both of their cultural broths and its organic extracts were assayed by antibacterial andnematicide model. Based on the morphological characteristics and biological activity,61strains were selected to carry out the16S rRNA sequences test. Results:100isolates were purified,43strains exhibited positive results in one or two screening model, the total positive rate was43%, the strain L-075and A-064exhibited positive results in six screening model,the16S rRNA sequences test showed that the61strains belongs to7different genera, including Streptomyces, Nocardiopsis, Pseudonocardia, Prauserella, Saccharopolyspora Halophilic, Actinomyces and Thermomonospora. The sequence similarity of strain L-068may be a potential new species.Conclusion:The studies demonstrated there are untapped actinomycetal resources e in hyperhaline region of Xingjang, China and its bioactive secondary metabolites deserved further exploration for microbial drugs.