Mapping Quantitative Trait Loci For Plant Height Related Characters In Wheat

Plant height is a complex and significant agronomic trait in wheat, which also has animportant impact to logding and yield. In this study, F2and F2:3populations both including230lines derived from the cross of line “0911-46” and line “42” were used to detect the genethat controlled plant height related traits in two environments in two years. The plant heightrelated characteristics contain plant height (PH), the internode length counted from the top(TL1), the internode length from the first to the third counted from the ground (GL1, GL2,GL3), the internode diameter from the first to the second counted from the ground (GD1,GD2)，GL1/PH, GL2/PH and GL1+GL2/PH. The main results of this work are as follows:1. The genetic linkage map348pairs of the polymorphism SSR primers between parents were obtained from1677pairs, and the polymorphism detection rate was20.75%. In F2:3populations,198pairs ofprimers performed polymorphism, accounting for56.90%of the polymorphism markers.Finally, a linkage map with176SSR makers covering the whole wheat genome wasconstructed. The linkage map covered1423.54cM with an average distance of8.09cM. Eachchain group had8.8markers except6A chromosome.2. Plant height QTL analysis16QTLs were detected for plant height in two environments in two years, Which werelocated on the chromosomes2B,2D,4B,4D,5A,6B,7A and7D and individual QTLexplained0.29%to63.42%of phenotypic variations. According to the field date of F2in YLin2011,7QTLs were detected; According to the field date of F2:3in YL and QX in2012, wedetected6and3QTLs for plant height respectively.3. Plant height related traits QTL analysis39QTLs for plant height related traits were detected using the field data in twoenvironments in two years, which were located on the chromosomes1A,1D,2A,2B,2D,3A,4B,4D,5A,5B,5D,6B,6D,7A and7B and singal QTL explained0.16%-45.08%ofphenotypic variations. There were11QTLs in F2populations in YL in2011, Detected17and11QTLs in F2:3populations in YL and QX in2012. In the maker interval of Xwmc473-Xwmc285on the chromosome4D, QTL for GL3was detected in the population ofF2:3in both YL and QX in2012, which showed stability of this QTL between environments.4. Plant height structures QTL analysis28QTLs of GL1/PH, GL2/PH, GL1+GL2/PH were detected using the field data in twoenvironments in two years, which were located on the chromosomes1A,1D,2B,2D,3A,3B,4A,5A,5B,5D,6B,6D,7A and7D and singal QTL explained0.02%-61.48%of phenotypicvariations. There were12QTLs in F2populations in YL in2011. Detected5and11QTL inF2:3populations in YL and QX in2012. In the maker interval of Xbarc148-Xcfd21onchromosome1D with genetic distances of0.01cM from marker Xbarc148, QTL for GL2/PHwere detected in both population of F2and F2:3in YL, which showed stability of this QTLbetween generations.5. Stem diameter QTL analysis12QTLs of stem diamter were detected using the field data in two environments in twoyears, which were located on the chromosomes1A,1D,3A,4A,4B,5A,5B,6B,7A and7Dand singal QTL could explain0.22%-15.51%of phenotypic variations. There was only1QTL in F2populations in YL in2011. There were5and6QTLs in F2:3populations in YL andQX in2012.