Effects Of Exogenous Co And No On Germination And Seedling Of Drought Stressed Xiao Yang22Wheat

Xiaoyang22wheat (Triticum aestivum Linn.) were treated with Hematin (CO donor) and sodium nitroprusside (SNP)(NO donor) to explore the effects of CO and NO on wheat growth, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein content and expression during wheat germination, seedling growth under drought stress48h. The research results will improve our understanding of NO and CO physiological function in drought-stressed plant. Therefore researching this problem has practical significance and theory value. The main results are as follows:1. Under drought stress, seed germination rate and germination potential reduced significantly, the content of malondialdehyde (MDA) increased significantly, the soluble protein and soluble sugar decreased, while the superoxide dismutase (SOD) activity was enhanced. In the period of early germination of wheat, exogenous NO and CO can increase seed germination rate and germination potential, Relative water content (RWC) increased significantly, MDA content decreased significantly, soluble protein and soluble sugar were increased, while the SOD enzyme activity was significantly enhanced. Exogenous NO and CO provide a good environment of cells for seed germination.2. At different stages of wheat seedlings in response to drought stress:RWC were significantly increased, MDA content decreased significantly, soluble protein and soluble sugar were increased, while the SOD enzyme activity was significantly enhanced. However, the performance of wheat in the period of three leaves is not the same as. This feature can be used to ensure wheat irrigation and signal substances time to saving water and ensuring the growth of wheat.3. Different concentrations of Hematin and SNP on wheat are different and has a stage. In different periods of wheat, the effect of0.1μmol·L-1Hematin and200μmol·L-1SNP are better. The growth of wheat showed that in the period of third leaves the effect of Hematin is better and in the period of second leaves the effect of SNP is better.4. The results of SDS-PAGE electrophoresis show that, in different periods of wheat, GAPDE can be induced by PEG. SNP and Hematin make the expression of GAPDH changed. Research shows that when the concentration of SNP was200μmol·L-1the expression of GAPDH is highest. When the concentration of Hematin was0.1μmol·L-1the expression of GAPDH is highest. However, all were lower than PEG group. Real-time fluorescence quantitative PCR also proved that the expression of GAPDH of wheat in the period of thrid leavess is increasesed. Compared with control group, the expression of GAPDH of wheat under drought stress are increasesed, and lower than PEG group.