Comparative Two Function-known Candidate Genes Expression Of Different Drought Tolerance Wheat Cultivars Under Water Deficit Stress

Transcription factors is playing an important role in the corresponding process of plantssubjected to environmental stress. After feeling stress signaling, transcription factor genesthrough regulating the upstream and downstream of a series of gene expression, resulting in aresponse to the protection of the protein, allowing plants to reduce stress injury and adapt tochanges in the external environment. Study of transcription factors is an effective way toreveal the mechanisms of plant resistance to adversity stress and cultivate resistance cropvarieties.According to the Wdreb2and Wlip19gene sequences, this study design PCR primers,using real-time quantitative PCR technology, analysis of10different genotype wheat underdrought stress expression of related genes.The results of real time quantitative PCR showsthat the expression trends of Wdreb2and Wlip19in different genotype wheat leaves subjectedto drought stress. Our study obtain these main conclusions:(1) Wdreb2and Wlip19regulate target gene as a positive regulatory factor after wheatunder drought stress. The expression of Wdreb2and Wlip19in wheat leaves are affected bydrought and the trend was upward, this suggests that Wdreb2and Wlip19play a moreimportant role during wheat against drought stress.(2) Wdreb2and Wlip19showing differential expression in different varieties of wheatleaves. Wdreb2and Wlip19expressed in drought tolerance in wheat leaves was significantlyhigher than in the drought sensitive wheat leaves. It explains Wdreb2and Wlip19presence ofgenotype-specific expression. After subjected to drought stress in wheat, expression ofWdreb2and Wlip19began to raise the amount to synthesize more transcription factors, incombination with cis-acting original, regulating the expression of genes in different drought,responding to drought stress.(3) Wdreb2and Wlip19showing differential stress response time in different varieties ofwheat leaves. Wdreb2in both of drought tolerance wheat and drought sensitive wheat forPEG stress response time are concentrated in6h, and Wlip19in drought tolerance wheat forPEG stress response time is earlier than drought sensitivity wheat, the time in droughttolerance wheat concentrated in3h, and in drought sensitivity wheat concentrated in6h. (4) Wdreb2and Wlip19showing differential expression trends in different varieties ofwheat leaves. The results showed that in drought tolerance wheat, Wdreb2appears twoexpression peaks in drought tolerance wheat, after the first expression quantity increases tothe peak, it started to come down to certain extent, that showed "up-down-up" trend;Wlip19appears one expression peak, after the hike to the peak, begin to cut, and tends to bestable. In drought sensitivity wheat, Wdreb2expression appeared one peaks; Wlip19expression appeared two peaks, showed a "raised-cut-raised" trend.